지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The replication competent adenoviral vector (AV), AdLPCDIRESE1A was generated and reported previously to have cytotoxic effects in some cell lines. In AdLPCDIRESE1A, the expression of cytosine deaminse (CD) and E1A genes are under the control of tumor-specific L-plastin promoter. CD enzyme can deaminate the nontoxic prodrug 5-fluorocytosine (5-FC) to the toxic 5-fluorouracil (5-FU). E1A gene is essential for viral replication. Primary liver cancer, most of which is hepatocellular carcinoma (HCC), is the third common leading cancer in Korea. Thus, we have conducted in vitro preclinical study to evaluate effectiveness of AdLPCDIRESE1A on HCC. The efficacy of cytotoxicity was measured by generation of cytopathic effect (CPE) and cell counting. We infected HepG2 cells with various MOI of vector alone or concurrent with 5-FC. Exposure of cells to AdLPCDIRESE1A generated a significant cytotoxic effect as compared to the control. Almost 83% of the cell had manifested the characteristic cytotoxic effect on day 9 after infection of cells with 10 MOI of vector. We also observed the additive cytotoxic effects when AdLPCDIRESE1A vector had been coadministrated with 5-FC. The results suggest that the use of AdLPCDIRESE1A/5FC may be value in treatment of liver cancer. Further animal studies are needed for clinical trial.
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참고문헌 (15)
참고문헌 신청
정인재 / 2003 / 암 유전자 치료제의 개발 현황 / 한국독성학회지 19(3):247~257
Chung, I / 1999 / Use of L-plastin promoter to develop an adenoviral system that confers transgene expression in ovarian cancer cells but not in normal mesothelial cells / ancer Gene Ther 6:99
Chung, I / 2004 / Recombinant adenoviral vector containing tumor-specific L-plastin promoter fused to cytosine deaminase gene as a transcription unit : generation and functional test / Arch. Pharm. Res 27:633
Lin, C.-S / 1993 / Human plastin genes / J. Biol. Chem 268:2781
Lin, C.-S / 1993 / Characterization of the human L-plastin gene promoter in normal and neoplastic cells / J. Biol. Chem 268:2793
Chung, I / 1999 / Use of L-plastin promoter to develop an adenoviral system that confers transgene expression in ovarian cancer cells but not in normal mesothelial cells / ancer Gene Ther 6:99
Chung, I / 2004 / Recombinant adenoviral vector containing tumor-specific L-plastin promoter fused to cytosine deaminase gene as a transcription unit : generation and functional test / Arch. Pharm. Res 27:633
Lin, C.-S / 1993 / Human plastin genes / J. Biol. Chem 268:2781
Lin, C.-S / 1993 / Characterization of the human L-plastin gene promoter in normal and neoplastic cells / J. Biol. Chem 268:2793
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