지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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In order to determine acetylcholine and choline in the biological samples, the specific enzymes of acetylcholinesterase (AChE) and choline oxidase (ChO), which utilize acetylcholine and choline as substrates, were employed to convert substrates to $H_2O_2$. The produced $H_2O_2$ was coupled to 4-aminoantipyrine/phenol with peroxidase (PO) yielding quinoneimine dye which was measured at 508 nm. In the present enzymatic spectrophotometric analysis the product at the equilibrium state was measured considering accuracy, precision, time and cost of the analysis. The developed analytical method yielded good linearity (calibration curve; $A_{508}$=9534[acetylcholine]+0.009, correlation coefficient ($R^2$); 0.999) with detection limit of $1.11{\times}10^{-7}M$, reasonable precision (relative standard deviation; 0.10~1.62% at $2.5{\times}10^{-6}M{\sim}1.25{\times}10^{-4}M$) and accuracy (relative error; -0.24~0.97% at $4.13{\times}10^{-6}M{\sim}1.01{\times}10^{-4}M$) for acetylcholine chloride standard solution. The concentrations of acetylcholine and choline in human serum were found as $3.20{\times}10^{-5}M$ and $1.14{\times}10^{-4}M$, respectively. The brain tissues of Sprague-Dawley strain rat contained 9.82${\mu}g/g$ of acetylcholine and 6.53 ${\mu}g/g$ of choline in the cerebrum, while 7.37 ${\mu}g/g$ of acetylcholine and 5.34 ${\mu}g/g$ of choline in the cerebellum.
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참고문헌 (22)
참고문헌 신청
Jamal, M. / 2005 / Inhibition of acetaldehyde metabolism decreases acetylcholine release in medial frontal cortex of freely moving rats / Brain Research 1039:90
Hrdina, P. D. / 1974 / Metabolism of brain acetylcholine and its modification by drugs / Drug Metab. Rev 3:89
Chang, Q. / 2003 / Switching memory system during learning: changes in patterns of brain acetylcholine release in the hippocampus and striatum in rats / J. Neurosci 23:3001
Uutela, P. / 2005 / Analysis of acetylcholine and choline in microdialysis samples by liquid chromatography/tandem mass spectrometry / Rapid Commun. Mass Spectrom 19:2950
Frolich, L. / 1998 / Acetylcholine in human CSF: methodological considerations and levels in dementia of Alzheimer type / J. Neural Transm 105:961
Hrdina, P. D. / 1974 / Metabolism of brain acetylcholine and its modification by drugs / Drug Metab. Rev 3:89
Chang, Q. / 2003 / Switching memory system during learning: changes in patterns of brain acetylcholine release in the hippocampus and striatum in rats / J. Neurosci 23:3001
Uutela, P. / 2005 / Analysis of acetylcholine and choline in microdialysis samples by liquid chromatography/tandem mass spectrometry / Rapid Commun. Mass Spectrom 19:2950
Frolich, L. / 1998 / Acetylcholine in human CSF: methodological considerations and levels in dementia of Alzheimer type / J. Neural Transm 105:961
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