지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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In this study, a high performance liquid chromatography-diode array detector method was established, for simultaneous determination of three compounds, berberine, palmatine and geniposide in Hwangryunhaedok-tang, To develop and validate method, $C_{18}$ column (5 ${\mu}M$, 4.6 mm${\times}$250 mm) was used with gradient mobile phase, water containing 0.1% trifluoroacetic acid (TFA) and MeOH at the column temperature of $30^{\circ}C$. UV wavelength was set at 230 and 280 nm. Validation of the chromatography method was evaluated by linearity, precision and accuracy test. Calibration curve of standard components showed good linearity ($R^2$ > 0.9999). The limits of detection (LOD) and limits of quantification (LOQ) varied from 0.05 to 0.17 ${\mu}g/ml$ and 0.15 to 0.53 ${\mu}g/ml$, respectively. The relative standard deviations (RSDs) data of intra-day and inter-day test were in less than 2.99% and 1.90%, respectively. The results of the accuracy test were in the range of 98.36 to 102.52% with RSDs values 0.32 to 1.98%. The results of validation indicated that this method was a very accurate and sensitive assay.
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참고문헌 (13)
참고문헌 신청
Normile, D / 2003 / Asian medicine. The new face of traditional Chinese medicine / Science 299:188
Jiang, W.-Y / 2005 / Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science / Trends. Pharmacol. Sci 26:558
Wang, Z. G / 2003 / Current status and future direction of Chinese herbal medicine / Trends. Pharmacol. Sci 23:347
Lu, A. P / 2004 / Theory of traditional Chinese medicine and therapeutic method of diseases / World J. Gastroenterol 10:1854
공민정 / 2004 / 황련해독탕(黃連解毒湯)과 및 가미방의 인지기능회복에 대한 연구 / 대한본초학회지 19(4):161~168
Jiang, W.-Y / 2005 / Therapeutic wisdom in traditional Chinese medicine: a perspective from modern science / Trends. Pharmacol. Sci 26:558
Wang, Z. G / 2003 / Current status and future direction of Chinese herbal medicine / Trends. Pharmacol. Sci 23:347
Lu, A. P / 2004 / Theory of traditional Chinese medicine and therapeutic method of diseases / World J. Gastroenterol 10:1854
공민정 / 2004 / 황련해독탕(黃連解毒湯)과 및 가미방의 인지기능회복에 대한 연구 / 대한본초학회지 19(4):161~168
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