Following-up changes in red blood cell deformability and membrane stability in the presence of PTFE graft implanted into the femoral artery in a canine model

Toth, Csaba; Kiss, Ferenc; Klarik, Zoltan; Gergely, Eszter; Toth, Eniko; Vanyolos, Katalin Peto Erzsebet; Miko, Iren; Nemeth, Norbert

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자료유형: 학술저널

저자정보: Toth, Csaba (Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen) Kiss, Ferenc (Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen) Klarik, Zoltan (Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen) Gergely, Eszter (Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen) Toth, Eniko (Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen) Vanyolos, Katalin Peto Erzsebet (Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen) Miko, Iren (Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen) Nemeth, Norbert (Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Deb)

저널정보: 한국유변학회 Korea-Australia rheology journal Korea-Australia rheology journal 제26권 제2호

발행연도: 2014.1

수록면: 209 - 215 (7page)

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It is known that a moderate mechanical stress can even improve the red blood cells' (RBC) micro-rheological characteristics, however, a more significant stress causes deterioration in the deformability. In this study, we aimed to investigate the effect of the presence of artificial graft on the RBC deformability and membrane stability in beagles. In the Control group only anesthesia was induced and in the postoperative (p.o.) period blood samplings were carried out. In the Grafted group under general anesthesia, the left femoral artery was isolated, from which a 3.5 cm segment was resected and a PTFE graft (O.D.: 3 mm) of equal in length was implanted into the gap. On the $1^{st}$, $3^{rd}$, $5^{th}$, $7^{th}$ and 14th p.o. days blood was collected the cephalic veins and RBC deformability was determined ektacytometry (LoRRca MaxSis Osmoscan). Membrane stability test consisted of two deformability measurements before and after the cells were being exposed to mechanical stress (60 or 100 Pa for 300 seconds). Compared to the Control group and the baseline values the red blood cell deformability showed significant deterioration on the $3^{rd}$, $5^{th}$ and mainly on the $7^{th}$ postoperative day after the graft implantation. The membrane stability of erythrocyte revealed marked inter-group difference on the $3^{rd}$, $5^{th}$ and $7^{th}$ day: in the Grafted group the deformability decreased and during the membrane stability test smaller difference was observed between the states before and after shearing. We concluded that the presence of a PTFE graft in the femoral artery may cause changes in RBC deformability in the first p.o. week. RBC membrane stability investigation shows a lower elongation index profile for the grafted group and a narrowed alteration in the deformability curves due to mechanical stress.

#red blood cell deformability #synthetic vascular graft #membrane stability test #mechanical trauma to red blood cell #animal model

참고문헌 (28)

참고문헌 신청

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