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(Laboratory of Alternative Medicine & Behavioral Neurosciences, Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University) (Laboratory of Alternative Medicine & Behavioral Neurosciences, Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University) (Laboratory of Alternative Medicine & Behavioral Neurosciences, Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University) (Laboratory of Alternative Medicine & Behavioral Neurosciences, Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University) (Laboratory of Alternative Medicine & Behavioral Neurosciences, Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, Jahangirnagar University) (Laboratory of Alternative Medicine & Behavioral Neurosciences, Department of Biochemistry and Molecular Biology, Faculty of Biological Scien)
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경희한의학연구센터 Oriental pharmacy and experimental medicine Oriental pharmacy and experimental medicine 제12권 제1호
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    초록·키워드

    We investigated the effect of Syzygium cumini (L.) Skeels seed extract on the oxidative stress of brain cortical tissues of alcohol-treated rats. The in vitro antioxidative effect of methnolic S. cumini seed extract was initially compared with those of the buytylated hydroxyl toluene (BHT) and Vitamin C, by determining their DPPH-free radical scavenging activity. The S. cumini seed extract exhibited stronger free radical scavenging activity than those of the BHT and Vitamin C. Cortex homogenates were then directly incubated with 15% ethanol and/or Fenton's reagent ($H_2O_2+Fe_2SO_4$) to induce in vitro oxidative stress in the absence or presence of S. cumini seed extract. The S. cumini seed extract significantly reduced the levels of lipid peroxide (LPO) in the cortical homogenates. Twenty four rats were then divided into four groups: Control, S. cumini seed extract (SE)-administered, 15% ethanol-fed (EtOH) and EtOH+SE rats. The oral administration of the extract (400 mg/kg BW.day) for 8 weeks significantly (P<0.05) decreased the levels of LPO in the cortex of the EtOH+SE rats, suggesting that S. cumini seed not only scavenged the DPPH-free radicals and obstructed the ethanol/Fenton's reagents-induced in vitro oxidative stress of the cortical tissues but also reduced their in vivo formation. These results suggest that S. cumini seed could be used as a potential antioxidant therapy for alcoholics.

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