지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 19 - 26 (8page)
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Objectives : Aucklandiae Radix (Muxiang) one of important herbal medicines in oriental medicine, is defined as the dried root of Aucklandia lappa (Asteraceae). Owing to the similarities in the morphology and name, Inulae Radix (Tu-Muxiang) and Vladimiriae Radix (Chuan-Muxiang) as well as Aristolochiae Radix (Qing-Muxiang) originated from other medicinal plants are often used as substitutes and/or adulterants of Aucklandiae Radix. Therefore, a reliable authentication of these herbal medicines is necessarily for the public health and prevention of misuse. Methods : 32 samples of medicinal plants supplying Aucklandiae Radix, Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix were collected in Korea and China. The ITS (Internal transcribed spacer) nucleotide sequences of samples were determined. The PCR primers to amply DNA marker of each herbal medicine were designed basing on the specific ITS regions showing differences in the sequences among medicinal plants. Results : Primer set Al R/IS F designed in this work amplified 220 bp PCR product only in samples of Aucklandiae Radix. In contrast, primer set Ih F/IS R, Vs R/IS F, and AcR F1/Ac R amplified 250 bp product, 356 bp prouct, and 516 bp product respectively to identify Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix. Conclusions : The primers designed basing on the nucleotide sequences of ITS regions appearing differenced in the sequences among medicinal plants amplified the DNA markers for the identification of Aucklandiae Radix, Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix. These herbal medicines were more efficiently identified by multiplex PCR method using all primers in a single PCR process.
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참고문헌 (35)
참고문헌 신청
China Pharmacopoeia Committee / 2005 / Pharmacopoeia of the People’s Republic of China / China Chemical Industry :41~42
Korea Food and Drug Administration / 2011 / The Korean Herbal Pharmacopoeia / Korea Food and Drug Administration press :133~134
Li AF / 2005 / Preparative isolation and purification of costunolide and dehydrocostuslactone from Aucklandia lappa Decne by high-speed counter- current chromatography / J Chromatogr A 1076(1):193~197
Chang HM / 1986 / But PPH. Pharmacology and applications of Chinese materia medica / World Scientific :190~194
Taniguchi M / 1995 / Costunolide and dehydrocostus lactone as inhibitors of killing function of cytotoxic T lymphocytes / Biosci Biotechnol Biochem 59:2064~2067
Korea Food and Drug Administration / 2011 / The Korean Herbal Pharmacopoeia / Korea Food and Drug Administration press :133~134
Li AF / 2005 / Preparative isolation and purification of costunolide and dehydrocostuslactone from Aucklandia lappa Decne by high-speed counter- current chromatography / J Chromatogr A 1076(1):193~197
Chang HM / 1986 / But PPH. Pharmacology and applications of Chinese materia medica / World Scientific :190~194
Taniguchi M / 1995 / Costunolide and dehydrocostus lactone as inhibitors of killing function of cytotoxic T lymphocytes / Biosci Biotechnol Biochem 59:2064~2067
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