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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 55 - 61 (7page)
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Objectives : Allium Hookeri (AH) is a traditional herb to treat inflammatory diseases in India and Myanmar. Recently, AH cultivation was succeeded in South Korea. This study was performed to evaluate the anti-inflammatory effects of Korean AH in RAW264.7 cells, mouse macrophage cell line. Methods : To evaluate the anti-inflammatory effects of root of AH, we prepared the 70% ethanol extract, then we examined the productions of nitrite, and pro-inflammatory cytokines. To examine the nitrite, and cytokines, the RAW264.7 cells were treated with AH, then stimulated with lipopolysaccharide (LPS, 500 ng/ml) for 24 h. Then the cells were harvested for griess assay, ELISA and real-time reverse transcription polymerase chain reaction (RT-PCR). Also to detect the ability of AH to induce heme oxygenase-1 (HO-1), we examined the HO-1 expression using real time RT-PCR and western blot. Furthermore, we examined the mitogen activated-protein kinases (MAPKs) and nuclear factor kappa B (NF-${\kappa}B$) activation to find out the underlying mechanisms. Results : AH ethanol extract significantly inhibited the productions of nitrite and interleukin (IL)-$1{\beta}$. AH treatment increased the HO-1 expression dramatically at 1 h, then peaked at 3 h. When the HO-1 was inhibited by tin (Sn) protoporphryin-IX (SnPP), the anti-inflammatory action of AH was reversed. AH treatment inhibited the activation of p38, but not extracelluar signal-regulated kinase (ERK 1/2) and c-Jun $NH_2$-terminal kinase (JNK) and also the degradation of inhibitory kappa B a (Ik-$B{\alpha}$) in the LPS-stimulated RAW 264.7 cells. Conclusions : These data could suggest that AH exerts anti-inflammatory influences through up-regulation of HO-1 and deactivation of p38.
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참고문헌 (36)
참고문헌 신청
Maines MD. / 1997 / The oxygenase system : a regular of second messenger gases / Annu Rev Pharmacol Toxicol 37:517~54
Zedler S. / 2006 / The impact of endogenous triggers on trauma associated inflammation / Curr Opin Crit Care 12:595~601
Vane JR. / 1994 / Inducible isoforms of cyclooxygenase and nitric oxide synthase in inflammation / Proc Natl Acad Sci U S A 91:2046~50
Ferrero-Miliani L. / 2007 / Chronic inflammation : importance of NOD2 and NALP3 in interleukin-1beta generation / Clin Exp Immunol 147(2):227~235
Hunt TK. / 1984 / Studies on inflammation and wound healing : angiogenesis and collagen synthesis stimulated in vivo by resident and activated wound macrophages / Surgery 96(1):48~54
Zedler S. / 2006 / The impact of endogenous triggers on trauma associated inflammation / Curr Opin Crit Care 12:595~601
Vane JR. / 1994 / Inducible isoforms of cyclooxygenase and nitric oxide synthase in inflammation / Proc Natl Acad Sci U S A 91:2046~50
Ferrero-Miliani L. / 2007 / Chronic inflammation : importance of NOD2 and NALP3 in interleukin-1beta generation / Clin Exp Immunol 147(2):227~235
Hunt TK. / 1984 / Studies on inflammation and wound healing : angiogenesis and collagen synthesis stimulated in vivo by resident and activated wound macrophages / Surgery 96(1):48~54
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