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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 87 - 93 (7page)
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Objectives : We recently have reported that constituents of OMC-2010 have an immuno-modulatory effects via inhibiting tumor necrosis factor (TNF)-alpha and interleukin (IL)-5. In this study, based on previous data, we investigated the effects of combinations with each OMC constituents on splenocyte cytotoxicity, cytokine productions, and ovalbumin (OVA) induced experimental allergic asthma. Methods : Mouse splenocytes were pre-treated with ethanol extract of constituents of Rehmannia glutinosa (RG), Pinellia ternata (PT), Schisandra chinensis (SC). We made 4 combinations using RG, PT, and SC (A;1:1:1, B;2:1:1, C;1:2:1, D;1:1:2). The cells were pretreated with A, B, C, or D for 1 h, then stimulated with lipopolysaccharide (LPS, $1{\mu}g/ml$) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokine productions. Then using effective combination from RG, PR and SC, we administrated the combination orally, then challenged with OVA to induce asthma. Then we analyzed the airway hyper-reactivity (AHR), lung histology and lung TNF-${\alpha}$ and IL-5 mRNA. Results : A. B. C. and D did not showed significant cytotoxicity on splenocytes. Pre-treatment of A inhibited the expression of TNF-${\alpha}$ and IL-5 significantly, but not B, C, and D. In experimental asthma, administration of A significantly inhibited the increase of AHR, lung damage, TNF-${\alpha}$ and IL-5 expression. Conclusions : Theses results could suggest that inhibitory effects of the ideal combination with RG, PT and SC (1:1:1) could be applied to treatment of asthma and study of asthma mechanisms.
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참고문헌 (23)
참고문헌 신청
Liu J / 2013 / Effects of taraxasterol on ovalbumin-induced allergic asthma in mice / J Ethnopharmacol 148(3):787~793
Bateman ED / 2008 / Global strategy for asthma management and prevention: GINA executive summary / Eur Respir J 31(1):143~178
Busse WW / 2001 / Asthma / N Engl J Med 344(5):350~362
Robinson DS / 1992 / Predominant Th2-like bronchoalveolar T-lymphocyte population in atopic asthma / N Engl J Med 326(5):298~304
Walker C / 1992 / Allergic and nonallergic asthmatics have distinct patterns of T-cell activation and cytokine production in peripheral blood and bronchoalvelora lavage / Am Rev Respir Dis 146(1):109~115
Bateman ED / 2008 / Global strategy for asthma management and prevention: GINA executive summary / Eur Respir J 31(1):143~178
Busse WW / 2001 / Asthma / N Engl J Med 344(5):350~362
Robinson DS / 1992 / Predominant Th2-like bronchoalveolar T-lymphocyte population in atopic asthma / N Engl J Med 326(5):298~304
Walker C / 1992 / Allergic and nonallergic asthmatics have distinct patterns of T-cell activation and cytokine production in peripheral blood and bronchoalvelora lavage / Am Rev Respir Dis 146(1):109~115
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