지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Rubisco is a major photosynthetic plant enzyme in the chloroplasts, catalyzing a photosynthetic reaction through carboxylation and oxygenation in the leaves. Despite its biological importance, its high abundance causes difficulties in the proper separation of protein mixtures during 2-dimensional gel electrophoresis (2-DE). Here, we resolved those plant soluble proteins by efficiently removing Rubisco. This resulted in a high quality and resolution of 2-DE gels. Rubisco removal was achieved through aggregation in the presence of a high DTT concentration, which subsequently increased the visualization of less abundant proteins and reduced horizontal streaking. This simple method may provide a means for finding more biologically important protein targets via plant proteomics.
목차
등록된 정보가 없습니다.
참고문헌 (12)
참고문헌 신청
Cánovas, FM / 2004 / Plant proteome analysis / Proteomics 4:285~298
Kyoungwon Cho / 2006 / Protein Extraction/Solubilization Protocol for Monocot and Dicot Plant Gel-Based Proteomics / Journal of Plant Biology(한국식물학회지) 49(6):413~1
Cortón M / 2004 / Improved resolution of the human adipose tissue proteome at alkaline and wide range pH by the addition of hydroxylethyl disulfide / Proteomics 4:438~441
Craven RA / 2002 / Increased protein entry together with improved focusing using a combined IPGphor/Multiphor approach / Proteomics 2:1061~1063
Greenough C / 2004 / A method for the rapid depletion of albumin and immunoglobin from human plasma / Proteomics 4:3107~3111
Kyoungwon Cho / 2006 / Protein Extraction/Solubilization Protocol for Monocot and Dicot Plant Gel-Based Proteomics / Journal of Plant Biology(한국식물학회지) 49(6):413~1
Cortón M / 2004 / Improved resolution of the human adipose tissue proteome at alkaline and wide range pH by the addition of hydroxylethyl disulfide / Proteomics 4:438~441
Craven RA / 2002 / Increased protein entry together with improved focusing using a combined IPGphor/Multiphor approach / Proteomics 2:1061~1063
Greenough C / 2004 / A method for the rapid depletion of albumin and immunoglobin from human plasma / Proteomics 4:3107~3111
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