지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 277 - 281 (5page)
이용수
초록· 키워드
오류제보하기
RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and vaginal secretion). While those makers successfully identified most body fluid samples, there were some cases of false positive and negative identification. To improve the accuracy of the identification further, we tried to use multiple markers per body fluid and adopted the NanoString nCounter system instead of a multiplex qRT-PCR system. After measuring tens of RNA markers, we evaluated the accuracy of each marker for body fluid identification. For body fluids, such as blood and semen, each body fluid-specific marker was accurate enough for perfect identification. However, for saliva and vaginal secretion, no single marker was perfect. Thus, we designed a logistic regression model with multiple markers for saliva and vaginal secretion and achieved almost perfect identification. In conclusion, the NanoString nCounter is an efficient platform for measuring multiple RNA markers per body fluid and will be useful for forensic RNA analysis.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Zubakov D / 2009 / New markers for old stains: stable mRNA markers for blood and saliva identification from up to 16-year-old stains / Int J Legal Med 123:71~74
Sakurada K / 2011 / Expression of statherin mRNA and protein in nasal and vaginal secretions / Leg Med 13:309~313
Haas C / 2011 / Selection of highly specific and sensitive mRNA biomarkers for the identification of blood / Forensic Sci Int Genet 5:449~458
Richard ML / 2012 / Evaluation of mRNA marker specificity for the identification of five human body fluids by capillary electrophoresis / Forensic Sci Int Genet 6:452~460
Haas C / 2009 / mRNA profiling for body fluid identification by reverse transcription endpoint PCR and realtime PCR / Forensic Sci Int Genet 3:80~88
Sakurada K / 2011 / Expression of statherin mRNA and protein in nasal and vaginal secretions / Leg Med 13:309~313
Haas C / 2011 / Selection of highly specific and sensitive mRNA biomarkers for the identification of blood / Forensic Sci Int Genet 5:449~458
Richard ML / 2012 / Evaluation of mRNA marker specificity for the identification of five human body fluids by capillary electrophoresis / Forensic Sci Int Genet 6:452~460
Haas C / 2009 / mRNA profiling for body fluid identification by reverse transcription endpoint PCR and realtime PCR / Forensic Sci Int Genet 3:80~88
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