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자료유형
학술저널
저자정보
Kim, Sang-Wook (Department of Animal Science, Chungbuk National University) Choi, Yang-Il (Department of Animal Science, Chungbuk National University) Choi, Jung-Suck (Department of Animal Science, Chungbuk National University) Kim, Jong-Joo (School of Biotechnology, Yeungnam University) Choi, Bong-Hwan (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) Kim, Tae-Hun (Division of Animal Genomics and Bioinformatics, National Institute of Animal Science) Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University)
저널정보
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제31권 제3호
발행연도
2011.1
수록면
356 - 365 (10page)

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We assessed the effects of single-nucleotide polymorphisms (SNPs) within the porcine fatty acid synthase (FASN) gene regarding meat quality and fatty acid composition in two pig populations: Korean native pigs (KNP) were crossed with Yorkshire (YS) $F_2$, and KNP were crossed with Landrace (LR) $F_2$. Direct DNA sequencing using eight KNP and eight YS pigs revealed two SNPs: c.265C>T (silent) in exon 4 and c.6545A>C (Asn${\rightarrow}$His) in exon 39. The frequency of the two SNPs was analyzed using the polymerase chain reaction-restriction fragment length polymorphism method in seven pig breeds and their association with meat quality traits and fatty acid composition was studied. In the $KNP{\times}YSF_2$ population, both SNPs were significantly associated with the level of monounsaturated fatty acids, including palmitoleic (C16:1) and oleic acid (C18:1) (p<0.005). c.6545A>C was associated with intramuscular fat content in both populations. Our results indicate that variations in c.265C>T and c.6545A>C of the pig FASN can be used to select animals with better fatty acid composition and meat quality. Moreover, KNP was a useful breed for identifying genetic factors affecting meat quality and fatty acid composition and for producing high quality pork.

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