siRNA-mediated Silencing of Notch-1 Enhances Docetaxel Induced Mitotic Arrest and Apoptosis in PCa Cells

Ye, Qi-Fa; Zhang, Yi-Chuan; Peng, Xiao-Qing; Long, Zhi; Ming, Ying-Zi; He, Le-Ye

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저자정보: Ye, Qi-Fa (Engineering and Technology Research Center for Transplantation Medicine of the National Ministry of Health) Zhang, Yi-Chuan (Engineering and Technology Research Center for Transplantation Medicine of the National Ministry of Health) Peng, Xiao-Qing (Department of Gastroenterology, The Third Xiangya Hospital, Central South University) Long, Zhi (Department of Urology, The Third Xiangya Hospital, Central South University) Ming, Ying-Zi (Engineering and Technology Research Center for Transplantation Medicine of the National Ministry of Health) He, Le-Ye (Department of Urology, The Third Xiangya Hospital, Central South University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제13권 제6호

발행연도: 2012.1

수록면: 2,485 - 2,489 (5page)

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Purpose: Notch is an important signaling pathway that regulates cell fate, stem cell maintenance and the initiation of differentiation in many tissues. It has been reported that activation of Notch-1 contributes to tumorigenesis. However, whether Notch signaling might have a role in chemoresistance of prostate cancer is unclear. This study aimed to investigate the effects of Notch-1 silencing on the sensitivity of prostate cancer cells to docetaxel treatment. Methods: siRNA against Notch-1 was transfected into PC-3 prostate cancer cells. Proliferation, apoptosis and cell cycle distribution were examined in the presence or absence of docetaxel by MTT and flow cytometry. Expression of $p21^{waf1/cip1}$ and Akt as well as activation of Akt in PC-3 cells were detected by Western blot and Real-time PCR. Results: Silencing of Notch-1 promoted docetaxel induced cell growth inhibition, apoptosis and cell cycle arrest in PC-3 cells. In addition, these effects were associated with increased $p21^{waf1/cip1}$ expression and decreased Akt expression and activation in PC-3 cells. Conclusion: Notch-1 promotes chemoresistance of prostate cancer and could be a potential therapeutic target.

#Notch signaling #prostate cancer #siRNA #cell cycle #apoptosis #$p21^{waf1/cip1}$ #Akt

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Ye, Qi-Fa

소속기관 Engineering and Technology Research Center for Transplantation Medicine of the National Ministry of