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논문 기본 정보

자료유형
학술저널
저자정보
Cai, Jun-Hong (College of Laboratory Medicine, Chongqing Medical University) Fu, Sheng-Miao (Department of Medicine Laboratory, Hainan Provincial People's Hospital) Tu, Zhi-Hua (Department of Medicine Laboratory, Hainan Provincial People's Hospital) Deng, Li-Qun (Department of Medicine Laboratory, Hainan Provincial People's Hospital) Liang, Zhu (Department of Medicine Laboratory, Hainan Provincial People's Hospital) Chen, Xin-Ping (Department of Medicine Laboratory, Hainan Provincial People's Hospital) Gong, Xuan-Ju (Department of Medicine Laboratory, Hainan Provincial People's Hospital) Wan, Li-Hua (College of Laboratory Medicine, Chongqing Medical University)
저널정보
아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제6호
발행연도
2015.1
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2,341 - 2,345 (5page)

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This study aimed to investigate the relationship between prognosis and protein and mRNA expression of an apoptotic inhibitor gene, survivin, in patients with nasopharyngeal carcinoma. Furthermore, functions of the survivin gene in the CNE2 nasopharyngeal carcinoma cell line were assessed. Immunohistochemistry and in situ hybridization were used in detecting the survivin protein and mRNA in 44 nasopharyngeal carcinoma specimens, and 30 chronic nasopharyngitis samples as controls. Survivin gene expression in CNE2 cell line was suppressed with an shRNA (short hairpin RNA). The positive ratios of expression for survivin protein and mRNA in nasopharyngeal carcinoma were 79.5% and 75.0% respectively, obviously higher than in the control group (p<0.01), and there is very good consistency between the two methods. The mean survival time of patients with higher survivin protein or mRNA expression was shorter than in patients with lower levelsv(p<0.01). Proliferation of the CNE2 cell line was distinctly inhibited by the shRNA. The results indicate that overexpression of the survivin gene plays an important role in onset and development of nasopharyngeal carcinoma, and it may be helpful for prognostic appraisal.

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