Effects of Rapamycin on Cell Apoptosis in MCF-7 Human Breast Cancer Cells

Tengku Din, Tengku Ahmad Damitri Al-Astani; Seeni, Azman; Khairi, Wirdatul-Nur Mohd; Shamsuddin, Shaharum; Jaafar, Hasnan

추천

검색

자료유형: 학술저널

저자정보: Tengku Din, Tengku Ahmad Damitri Al-Astani (Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia) Seeni, Azman (Advanced Medical and Dental Institute, Universiti Sains Malaysia) Khairi, Wirdatul-Nur Mohd (Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia) Shamsuddin, Shaharum (School of Health Sciences, Universiti Sains Malaysia) Jaafar, Hasnan (Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제24호

발행연도: 2014.1

수록면: 10,659 - 10,663 (5page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: Rapamycin is an effective anti-angiogenic drug. However, the mode of its action remains unclear. Therefore, in this study, we aimed to elucidate the antitumor mechanism of rapamycin, hypothetically via apoptotic promotion, using MCF-7 breast cancer cells. Materials and Methods: MCF-7 cells were plated at a density of $1{\times}10^5$ cells/well in 6-well plates. After 24h, cells were treated with a series of concentrations of rapamycin while only adding DMEM medium with PEG for the control regiment and grown at $37^{\circ}C$, 5% $CO_2$ and 95% air for 72h. Trypan blue was used to determine the cell viability and proliferation. Untreated and rapamycin-treated MCF-7 cells were also examined for morphological changes with an inverted-phase contrast microscope. Alteration in cell morphology was ascertained, along with a stage in the cell cycle and proliferation. In addition, cytotoxicity testing was performed using normal mouse breast mammary pads. Results: Our results clearly showed that rapamycin exhibited inhibitory activity on MCF-7 cell lines. The $IC_{50}$ value of rapamycin on the MCF-7 cells was determined as $0.4{\mu}g/ml$ (p<0.05). Direct observation by inverted microscopy demonstrated that the MCF-7 cells treated with rapamycin showed characteristic features of apoptosis including cell shrinkage, vascularization and autophagy. Cells underwent early apoptosis up to 24% after 72h. Analysis of the cell cycle showed an increase in the G0G1 phase cell population and a corresponding decrease in the S and G2M phase populations, from 81.5% to 91.3% and 17.3% to 7.9%, respectively. Conclusions: This study demonstrated that rapamycin may potentially act as an anti-cancer agent via the inhibition of growth with some morphological changes of the MCF-7 cancer cells, arrest cell cycle progression at G0/G1 phase and induction of apoptosis in late stage of apoptosis. Further studies are needed to further characterize the mode of action of rapamycin as an anti-cancer agent.

#Rapamycin #MCF-7 breast cancer cells #apoptosis

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요