The Anti-Inflammatory Activity of Eucommia ulmoides Oliv. Bark. Involves NF-κB Suppression and Nrf2-Dependent HO-1 Induction in BV-2 Microglial Cells

Kwon, Seung-Hwan; Ma, Shi-Xun; Hwang, Ji-Young; Ko, Yong-Hyun; Seo, Ji-Yeon; Lee, Bo-Ram; Lee, Seok-Yong; Jang, Choon-Gon

추천

검색

자료유형: 학술저널

저자정보: Kwon, Seung-Hwan (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) Ma, Shi-Xun (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) Hwang, Ji-Young (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) Ko, Yong-Hyun (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) Seo, Ji-Yeon (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) Lee, Bo-Ram (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) Lee, Seok-Yong (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University) Jang, Choon-Gon (Department of Pharmacology, School of Pharmacy, Sungkyunkwan University)

저널정보: 한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제24권 제3호

발행연도: 2016.1

수록면: 268 - 282 (15page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

In the present study, we investigated the anti-inflammatory properties of Eucommia ulmoides Oliv. Bark. (EUE) in lipopolysaccharide (LPS)-stimulated microglial BV-2 cells and found that EUE inhibited LPS-mediated up-regulation of pro-inflammatory response factors. In addition, EUE inhibited the elevated production of pro-inflammatory cytokines, mediators, and reactive oxygen species (ROS) in LPS-stimulated BV-2 microglial cells. Subsequent mechanistic studies revealed that EUE suppressed LPS-induced phosphorylation of mitogen-activated protein kinases (MAPKs), phosphoinositide-3-kinase (PI3K)/Akt, glycogen synthase $kinase-3{\beta}$ ($GSK-3{\beta}$), and their downstream transcription factor, nuclear factor-kappa B ($NF-{\kappa}B$). EUE also blocked the nuclear translocation of $NF-{\kappa}B$ and inhibited its binding to DNA. We next demonstrated that EUE induced the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulated heme oxygenase-1 (HO-1) expression. We determined that the significant up-regulation of HO-1 expression by EUE was a consequence of Nrf2 nuclear translocation; furthermore, EUE increased the DNA binding of Nrf2. In contrast, zinc protoporphyrin (ZnPP), a specific HO-1 inhibitor, blocked the ability of EUE to inhibit NO and $PGE_2$ production, indicating the vital role of HO-1. Overall, our results indicate that EUE inhibits pro-inflammatory responses by modulating MAPKs, PI3K/Akt, and $GSK-3{\beta}$, consequently suppressing $NF-{\kappa}B$ activation and inducing Nrf2-dependent HO-1 activation.

#Eucommia ulmoides Oliv #Bark #Pro-inflammatory responses #Nuclear factor-kappa B #Nuclear factor erythroid 2-related factor 2 #Heme oxygenase-1 #BV-2 microglial cells

참고문헌 (56)

참고문헌 신청

Amor, S / 2010 / Inflammation in neurodegenerative diseases / Immunology 129:154~169

Baima, E. T / 2010 / Novel insights into the cellular mechanisms of the anti-inflammatory effects of NF-kB essential modulator binding domain peptides / J. Biol. Chem 285:13498~13506

Bauer, M / 2002 / Heme oxygenase-1: redox regulation and role in the hepatic response to oxidative stress / Antioxid. Redox Signal 4:749~758

Block, M. L / 2005 / Microglia and inflammation-mediated neurodegeneration: multiple triggers with a common mechanism / Prog. Neurobiol 76:77~98

Block, M. L / 2007 / Microglia-mediated neurotoxicity:uncovering the molecular mechanisms / Nat. Rev. Neurosci 8:57~69

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!