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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 451 - 459 (9page)
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초록· 키워드
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Peroxiredoxins (Prxs) have a critical role in protecting cells against oxidative damage generated by reactive oxygen species (ROS). PrxI and PrxII are more than 90% homologous in their amino acid sequences, and both proteins reduce $H_2O_2$. In this study, an over-expression plasmid carrying PrxI was transfected into $PrxII^{-/-}$ mouse embryonic fibroblasts (MEFs) to investigate potential compensatory relationships between PrxI and PrxII. ROS levels induced by oxidative stress were increased in $PrxII^{-/-}$ MEFs as compared to wild-type MEFs. Moreover, exposure of $PrxII^{-/-}$ MEFs to $H_2O_2$ caused a reduction in cell viability of about 10%, and the proportion of cell death was increased compared to mock-treated $PrxII^{-/-}$ MEFs. However, transient over-expression of PrxI in $PrxII^{-/-}$ MEFs conferred increased resistance against the oxidative damage, as evidenced by increased cell viability and reduced intracellular ROS levels under $H_2O_2$ stress conditions. The findings suggest that over-expressed PrxI can partly compensate for the loss of PrxII function in PrxII-deficient MEFs.
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참고문헌 (27)
참고문헌 신청
Chae, H. Z / 1999 / Characterization of three isoforms of mammalian peroxiredoxin that reduce peroxides in the presence of thioredoxin / Diabetes Res. Clin. Pract 45:101~112
Chae, H. Z / 1993 / Cloning, sequencing, and mutation of thiol-specifi c antioxidant gene of Saccharomyces cerevisiae. / J. Biol. Chem 268:16815~16821
Claiborne, A / 2001 / Structural, redox, and mechanistic parameters for cysteine- sulfenic acid function in catalysis and regulation / Adv. Protein Chem 58:215~276
Han, Y. H / 2005 / Inhibitory role of peroxiredoxin II (Prx II) on cellular senescence (FEBS 29846) (vol 579, pg 4897, 2005) / FEBS LETTERS 579:5112~5112
Iwahara, S / 1995 / Purifi cation, characterization, and cloning of a heme-binding protein (23 kDa) in rat liver cytosol / Biochemistry 34:13398~13406
Chae, H. Z / 1993 / Cloning, sequencing, and mutation of thiol-specifi c antioxidant gene of Saccharomyces cerevisiae. / J. Biol. Chem 268:16815~16821
Claiborne, A / 2001 / Structural, redox, and mechanistic parameters for cysteine- sulfenic acid function in catalysis and regulation / Adv. Protein Chem 58:215~276
Han, Y. H / 2005 / Inhibitory role of peroxiredoxin II (Prx II) on cellular senescence (FEBS 29846) (vol 579, pg 4897, 2005) / FEBS LETTERS 579:5112~5112
Iwahara, S / 1995 / Purifi cation, characterization, and cloning of a heme-binding protein (23 kDa) in rat liver cytosol / Biochemistry 34:13398~13406
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