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논문 기본 정보

자료유형
학술저널
저자정보
Lim, Won-Suk (Department of Dermatology, Dongguk University Ilsan Hospital) Kim, Chang-Hyun (Department of Dermatology, Dongguk University Ilsan Hospital) Kim, Ji-Young (Department of Dermatology, Dongguk University Ilsan Hospital) Do, Byung-Rok (Biotechnology Research Institute, Hurim BioCell Inc.) Kim, Eo Jin (Department of Pathology, Dongguk University Ilsan Hospital) Lee, Ai-Young (Department of Dermatology, Dongguk University Ilsan Hospital)
저널정보
한국응용약물학회 Biomolecules & Therapeutics(구 응용약물학회지) Biomolecules & therapeutics 제22권 제4호
발행연도
2014.1
수록면
328 - 333 (6page)

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Vitiligo is a pigmentary disorder induced by a loss of melanocytes. In addition to replacement of pure melanocytes, cocultures of melanocytes with keratinocytes have been used to improve the repigmentation outcome in vitiligo treatment. We previously identified by in vitro studies, that adipose-derived stem cells (ADSCs) could be a potential substitute for keratinocytes in cocultures with melanocytes. In this study, the efficacy of pigmentation including durability of grafted melanocytes and short-term safety was examined in the nude mouse and Sprague-Dawley rat after grafting of primary cultured human melanocytes, with or without different ratios of primary cultured human ADSCs. Simultaneous grafting of melanocytes and ADSCs, which were separately cultured and mixed on grafting at the ratios of 1:1, 1:2, or 1:3, showed better efficacy than that of pure melanocytes. Grafting of melanocytes cocultured with ADSCs resulted in a similar outcome as the grafting of cell mixtures. Skin pigmentation by melanocytes : ADSCs at the ratios of 1:1 and 1:2 was better than at 1:3. No significant difference was observed between the 1-week and 2-week durations in coculturing. Time-course microscopic examination showed that the grafted melanocytes remained a little longer than 6-week post-grafting. No inflammatory cell infiltration was observed in the grafted skin and no melanocytes were detectable in other organs. Collectively, grafting of melanocytes and ADSCs was equally safe and more effective than grafting of melanocytes alone. Despite the absence of significant differences in efficacy between the group of 1:1 and that of 1:2 ratio, 1:2 ratio for 1-week coculturing may be better for clinical use from the cost-benefit viewpoint.
#Melanocytes #Adipose-derived stem cells #Grafting #Animal #Efficacy #Short-term safety

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참고문헌 (18)

참고문헌 신청
Austin, L. M / 1992 / The detection of melanocyte autoantibodies in the Smyth chickenmodel for vitiligo / Clin. Immunol. Immunopathol 64:112~120 google schola Brysk, M. M / 1989 / Repigmentation of vitiliginousskin by cultured cells / Pigment. Cell Res 2:202~207 google schola Falabella, R / 1989 / Transplantation ofin vitro-cultured epidermis bearing melanocytes for repigmentingvitiligo / J. Am. Acad. Dermatol 21:257~264 google schola Germain, L / 1995 / Earlybasement membrane formation following the grafting of culturedepidermal sheets detached with thermolysin or Dispase / Burns 21:175~180 google schola Guerra, L / 2000 / Treatmentof "stable" vitiligo by Timedsurgery and transplantation of culturedepidermal autografts / Arch. Dermatol 136:1380~1389 google schola

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