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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제57권 제2호
- 발행연도
- 2014.1
- 수록면
- 113 - 122 (10page)
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A5E is complex of several medicinal herb ethanol extracts. The aim of this study is investigating the anticancer effect for non-small cell lung cancer. The antitumor effects of A5E on NCI-H460 were examined by regulation of cell proliferation, apoptosis, cell cycle arrest, mitochondrial membrane potential (${\Delta}{\Psi}_m$), and apoptosis-related protein. Cell proliferation was measured by MTS assay. Apoptosis induced by A5E was confirmed by Annexin V-fluorescein isothiocyanate (FITC)/Propidium Iodide (PI) staining, and cell cycle arrest was measured by PI staining. NF-${\kappa}B$ translocation was detected by immunofluorescence and MMP (${\Delta}{\Psi}_m$) was measured by JC-1 staining. The expression of extrinsic pathway molecules such as FasL and FADD were elevated, and procaspase-8 was processed by A5E. In addition, intrinsic pathway related molecules were altered. The Bcl-2 and Bcl-xl levels decreased, Bax increased, and cytochrome C was released. In addition, the mitochondrial membrane potential collapsed, and caspase-3 and poly-(ADP-ribose) polymerase were processed by A5E. Moreover, A5E affected the cellular survival pathway involving phosphatidylinositol 3-kinase (PI3K)/Akt and NF-${\kappa}B$. PI3K and Akt were downregulated, also NF-${\kappa}B$ expression was decreased, and nuclear translocalization was inhibited by A5E. These results suggested that A5E delays proliferation, inhibit cell cycle progression and induce apoptosis in human lung cancer cell. We conclude that A5E is a potential anticancer agent for human lung carcinoma.
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참고문헌 (37)
참고문헌 신청
Allen RT / 1997 / Morphological and biochemical characterization and analysis of apoptosis / Pharmacol Tox Met 37:215~228
Astanehe A / 2008 / Mechanisms underlying p53 regulation of PIK3CA transcription in ovarian surface epithelium and in ovarian cancer / J Cell Sci 121:664~674
Bak Y / 2013 / A1E inhibits proliferation and induces apoptosis in NCI-H460 lung cancer cells via extrinsic and intrinsic pathways / Mol Bio Rep 40:4507~4519
Brennan P / 1997 / Phosphatidylinositol 3-kinase couples the interleukin-2 receptor to the cell cycle regulator E2F / Immunity 7:679~689
Calviello G / 2003 / gamma-Tocopheryl quinone induces apoptosis in cancer cells via caspase-9 activation and cytochrome c release / Carcinogenesis 24:427~433
Astanehe A / 2008 / Mechanisms underlying p53 regulation of PIK3CA transcription in ovarian surface epithelium and in ovarian cancer / J Cell Sci 121:664~674
Bak Y / 2013 / A1E inhibits proliferation and induces apoptosis in NCI-H460 lung cancer cells via extrinsic and intrinsic pathways / Mol Bio Rep 40:4507~4519
Brennan P / 1997 / Phosphatidylinositol 3-kinase couples the interleukin-2 receptor to the cell cycle regulator E2F / Immunity 7:679~689
Calviello G / 2003 / gamma-Tocopheryl quinone induces apoptosis in cancer cells via caspase-9 activation and cytochrome c release / Carcinogenesis 24:427~433
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