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논문 기본 정보

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저자정보
Balakrishnan, Bijinu (Department of Biological Sciences and Bioinformatics, Myongji University) Lim, Yoon Ji (Department of Biological Sciences and Bioinformatics, Myongji University) Hwang, Seok Hyun (Department of Biological Sciences and Bioinformatics, Myongji University) Lee, Doh Won (Department of Biological Sciences and Bioinformatics, Myongji University) Park, Si-Hyung (Department of Oriental Medicine Resources and Institute for Traditional Korean Medicine Industry, Mokpo National University) Kwon, Hyung-Jin (Department of Biological Sciences and Bioinformatics, Myongji University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제60권 제3호
발행연도
2017.1
수록면
249 - 256 (8page)

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The Monascus azaphilone (MAz) pigment is a well-known food colorant that has yellow, orange and red components. The structures of the yellow and orange MAz differ by two hydride reductions, with yellow MAz being the reduced form. Orange MAz can be non-enzymatically converted to red MAz in the presence of amine derivatives. It was previously demonstrated that mppE and mppG are involved in the biosynthesis of yellow and orange MAz, respectively. However, ${\Delta}mppE$ and ${\Delta}mppG$ knockout mutants maintained residual production of yellow and orange MAz, respectively. In this study, we deleted the region encompassing mppD, mppE and mppG in M. purpureus and compared the phenotype of the resulting mutant (${\Delta}mppDEG$) with that of an mppD knockout mutant (${\Delta}mppD$). It was previously reported that the ${\Delta}mppD$ strain retained the ability to produce MAz but at approximately 10% of the level observed in the wildtype strain. A chemical analysis demonstrated that the ${\Delta}mppDEG$ strain was still capable of producing both yellow and orange MAz, suggesting the presence of minor MAz route(s) not involving mppE or mppG. Unexpectedly, the ${\Delta}mppDEG$ strain was observed to accumulate fast-eluting pigments in a reverse phase high-performance liquid chromatography analysis. A LC-MS analysis identified these pigments as ethanolamine derivatives of red MAz, which had been previously identified in an mppE knockout mutant that produces high amounts of orange MAz. Although the underlying mechanism is largely unknown, this study has yielded an M. purpureus strain that selectively accumulates red MAz.
#Azaphilone pigment #Monascus purpureus #Phenotypic characterization of mppDEG deletion mutant #Selective production of red azaphilone pigments

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참고문헌 (21)

참고문헌 신청
Bijinu Balakrishnan / 2015 / A New Protein Factor in the Product Formation of Non-Reducing Fungal Polyketide Synthase with a C-Terminus Reductive Domain / Journal of Microbiology and Biotechnology 25(10):1648~1652 Crossref Balakrishnan B / 2016 / Delineating citrinin biosynthesis: Ctn-ORF3 dioxygenase-mediated multi-step methyl oxidation precedes a reduction-mediated pyran ring cyclization / Bioorg Med Chem Lett 26:392~396 google schola Balakrishnan B / 2014 / Mpp7 controls regioselective Knoevenagel condensation during the biosynthesis of Monascus azaphilone pigments / Tetrahedron Lett 55:1640~1643 google schola Balakrishnan B / 2013 / Genetic localization and in vivo characterization of a Monascus azaphilone pigment biosynthetic gene cluster / Appl Microbiol Biotechnol 97:6337~6345 google schola Bijinu Balakrishnan / 2014 / Monascus Azaphilone Pigment Biosynthesis Employs a Dedicated Fatty Acid Synthase for Short Chain Fatty Acyl Moieties / Applied Biological Chemistry 57(2):191~196 google schola

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