지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 678 - 684 (7page)
이용수
초록· 키워드
오류제보하기
To establish a system that facilitates the coordinate expression of multiple genes in a crop biotechnology setting, we tested the internal ribosome entry site (IRES) sequence from encephalimyocarditis virus (an animal viral polycistronic sequence) in rice plants. Three reporter genes, green fluorescent protein (Gfp), ${\beta}$-glucuronidase (Gus) and luciferase (Luc), were linked to IRES under the control of the ubiquitin promoter, generating FIG (G$\underline{fp}$-$\underline{I}$RES-$\underline{G}$us) and FIGIL (G$\underline{fp}$-$\underline{I}$RES-$\underline{G}$us-$\underline{I}$RES-$\underline{L}$uc) constructs, respectively. We obtained a total 38 transgenic lines using these vectors (24 for FIC and 14 for FIGIL) and the transgene integration and copy numbers for each were determined by Southern blot analysis. By northern blot analysis using Gfp, Gus and Luc probes, both the FIG and FIGIL rice plants showed multiple truncated transcripts, including full-length recombinant genes. Western blot analyses using GFP and GUS antibodies revealed the accumulation of GFP but not GUS protein in both FIG and FIGIL rice leaf tissues. Subsequent GFP and GUS reporter expression patterns showed GFP fluorescence but not GUS-staining in both FIG and FIGIL transgenic rice callus tissues. These results suggest that the direct application of animal viral IRES sequences is not sufficient to induce polycistronic expression in rice plants due to the production of multiple truncated transcripts.
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