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논문 기본 정보

자료유형
학술저널
저자정보
Liu, Shu Yi (School of Clinical Medicine, Xi'an Medical University) Liu, Sheng Ze (Department of Neurosurgery, Fuzhou Second Affiliated Hospital of Xiamen University) Li, Yu (Department of Otolaryngology, Second Affiliated Hospital of Xi'an Medical University) Chen, Shi (Department of Neurosurgery, Fuzhou Second Affiliated Hospital of Xiamen University)
저널정보
대한신경외과학회 대한신경외과학회지 대한신경외과학회지 제62권 제6호
발행연도
2019.1
수록면
626 - 634 (9page)

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Objective : Nerve growth factor (NGF) is a member of the neurotrophic factor family and plays a vital role in the physiological processes of organisms, especially in the nervous system. Many recent studies have reported that NGF is also involved in the regulation of tumourigenesis by either promoting or suppressing tumor growth, which depends on the location and type of tumor. However, little is known regarding the effect of NGF on interspinal schwannoma (IS). In the present study, we aimed to explored whether mouse nerve growth factor (mNGF), which is widely used in the clinic, can influence the growth of interspinal schwannoma cells (ISCs) isolated from IS in vitro. Methods : ISCs were isolated, cultured and identified by S-100 with immunofluorescence analysis. S-100-positive cells were divided into five groups, and separately cultured with various concentrations of mNGF (0 [phosphate buffered saline, PBS], 40, 80, 160, and 320 ng/mL) for 24 hours. Western blot and quantantive real time polymerase chain reaction (PCR) were applied to detect tyrosine kinase A (TrkA) receptor and p75 neurotrophin receptor ($p75^{NTR}$) in each group. Crystal violet staining was selected to assess the effect of mNGF (160 ng/mL) on ISCs growth. Results : ISCs growth was enhanced by mNGF in a dose-dependent manner. The result of crystal violet staining revealed that it was significantly strengthened the cells growth kinetics when cultured with 160 ng/mL mNGF compared to PBS group. Western blot and quantantive real time PCR discovered that TrkA receptor and mRNA expression were both up-regualated under the condition of mNGF, expecially in 160 ng/mL, while the exoression of $p75^{NTR}$ demonstrated no difference among groups. Conclusion : From these data, we conclude that exogenous mNGF can facilitate ISC growth by activating both TrkA receptor and $p75^{NTR}$. In addition, patients who are suffering from IS should not be administered mNGF in the clinic.
#Schwannoma #Mouse nerve growth factor #TrkA #$p75^{NTR}$

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참고문헌 (37)

참고문헌 신청
Ahmad I / 2014 / p75NTR is highly expressed in vestibular schwannomas and promotes cell survival by activating nuclear transcription factor kappa B / Glia 62:1699~1712 google schola Chattopadhyay S / 2009 / MMP-9 controls Schwann cell proliferation and phenotypic remodeling via IGF-1 and ErbB receptor-mediated activation of MEK/ERK pathway / Glia 57:1316~1325 google schola Chou TT / 2000 / A novel apoptotic pathway induced by nerve growth factor-mediated TrkA activation in medulloblastoma / J Biol Chem 275:565~570 google schola Davidson B / 2003 / Expression and activation of the nerve growth factor receptor TrkA in serous ovarian carcinoma / Clin Cancer Res 9:2248~2259 google schola Friedman WJ / 2010 / Proneurotrophins, seizures, and neuronal apoptosis / Neuroscientist 16:244~252 google schola

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