Inhibitory Effects of Ethanol Extracts from Nuruk on Oxidative Stress, Melanogenesis, and Photo-Aging

Lee, Sang-Jin; Cho, Sung-Won; Kwon, Yi-Young; Kwon, Hee-Suk; Shin, Woo-Chang

추천

검색

자료유형: 학술저널

저자정보: Lee, Sang-Jin (Research Institute, Kooksoondang Brewery Co., Ltd.) Cho, Sung-Won (Research Institute, Kooksoondang Brewery Co., Ltd.) Kwon, Yi-Young (Research Institute, Kooksoondang Brewery Co., Ltd.) Kwon, Hee-Suk (Research Institute, Kooksoondang Brewery Co., Ltd.) Shin, Woo-Chang (Research Institute, Kooksoondang Brewery Co., Ltd.)

저널정보: 한국균학회 Mycobiology Mycobiology 제40권 제2호

발행연도: 2012.1

수록면: 117 - 123 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Nuruk contributes to the unique characteristics of Korean alcoholic beverages. In this study, the effects of nuruk extracts (NE) on anti-oxidant characters, melanogenesis, and anti-photoaging activity were investigated. NEs were obtained from the 70% ethanol extracts of six types of nuruk, which have been used in brewing of fermented alcohol beverages in Korea. First, various antioxidant characteristics were identified in terms of 2,2'-azino-bis(3-ethylbenzthiozoline-6-sulphonic acid) (ABTS) radical scavenging activity, superoxide dismutase (SOD) expression, and inhibition of xanthine oxidase activity. NE#4 exhibited potent ABTS radical scavenging activity ($IC_{50}$ = 19.51 ${\mu}g$/mL). Compared with NE#4, relatively lower levels of activity were observed for NE#3 and NE#6, with $IC_{50}$ values of 90.99 and 76.88 ${\mu}g$/mL, respectively. According to results of western blot analysis for determination of SOD expression in $H_2O_2$-treated HepG2 cells, NE#5 and NE#6 induced a dramatic increase in the expression ratio of SOD, compared to the group treated with $H_2O_2$ only. Activity of xanthine oxidase, which converts xanthine into uric acid, generating superoxide ions, was inhibited by NE#4 and NE#6 in a dose-dependent manner. NE#4 induced significant inhibition of mushroom tyrosinase activity. A reduction in cellular melanin contents of 80% was observed in B16F1 melanocytes treated with NE#5 and NE#6; these effects were similar to those of arbutin at 100 ${\mu}M$. In addition, gelatin zymography and reverse transcription-PCR analysis were performed for assessment of anti-photoaging activity of Nuruk. Treatment with NE#6 resulted in dramatically inhibited activities of matrix metalloproteinase (MMP)-2/-9, suppressed expression of MMP-1, and increased expression of type-1 procollagen. Results of gelatin zymography for NE#4 and NE#5 were similar, to a slightly lesser degree. These results suggest the potential of NE#4 and NE#6 as natural ingredients for use in functional foods and cosmetics.

#Anti-oxidant activity #Anti-photoaging #Melanogenesis #Nuruk

참고문헌 (27)

참고문헌 신청

Shon SK / 1990 / Takju brewing of uncooked rice starch using Rhizopus koj / Korean J Appl Microbiol Biotechnol 18:506~510

YU, TAE SHICK / 2004 / A New Species of Hyphomycetes, Aspergillus coreanus sp. nov., Isolatedfrom Traditional Korean Nuruk / Journal of Microbiology and Biotechnology 14(1):182~187

Siyoung Yang / 2011 / Fungi Associated with the Traditional Starter Cultures Used for Rice Wine in Korea / Journal of the Korean Society for Applied Biological Chemistry 54(6):933~943

Kim HR / 2001 / Research trend of Korean traditional alcoholic beverage / Food Ind Nutr 6:5~10

Hye-Ryun Kim / 2011 / Identification and Characterization of Useful Fungi with α-Amylase Activity from the Korean Traditional Nuruk / Mycobiology 39(4):278~282