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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 272 - 279 (8page)
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To screen molecular chaperones similar to small heat shock proteins (sHsps), but without ${\alpha}$-crystalline domain, heat-stable proteins from Schizosaccharomyces pombe were analyzed by 2-dimensional electrophoresis and matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Sixteen proteins were identified, and four recombinant proteins, including cofilin, NTF2, pyridoxin biosynthesis protein (Snz1) and Wos2 that has an ${\alpha}$-crystalline domain, were purified. Among these proteins, only Snz1 showed the anti-aggregation activity against thermal denaturation of citrate synthase. However, pre-heating of NTF2 and Wos2 at $70^{\circ}C$ for 30 min, efficiently prevented thermal aggregation of citrate synthase. These results indicate that Snz1 and NTF2 possess molecular chaperone activity similar to sHsps, even though there is no ${\alpha}$-crystalline domain in their sequences.
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참고문헌 (30)
참고문헌 신청
Fu X / 2014 / Chaperone function and mechanism of small heatshock proteins / Acta Biochim Biophys Sin 46:347~356
Saibil H / 2013 / Chaperone machines for protein folding, unfolding and disaggregation / Nat Rev Mol Cell Biol 14:630~642
Ahn J / 2012 / Small heat-shock protein Hsp9has dual functions in stress adaptation and stress-induced G2-M checkpoint regulation via Cdc25 inactivation in Schizosaccharomyces pombe / Biochem Biophys Res Commun 417:613~618
Santino A / 2012 / Hsp90 interaction with Cdc2 and Plo1 kinases contributes to actomyosin ring condensation in fission yeast / Curr Genet 58:191~203
Morrow G / 2015 / Drosophila melanogaster Hsp22: a mitochondrial small heat shock protein influencing the aging process / Front Genet 6:1026
Saibil H / 2013 / Chaperone machines for protein folding, unfolding and disaggregation / Nat Rev Mol Cell Biol 14:630~642
Ahn J / 2012 / Small heat-shock protein Hsp9has dual functions in stress adaptation and stress-induced G2-M checkpoint regulation via Cdc25 inactivation in Schizosaccharomyces pombe / Biochem Biophys Res Commun 417:613~618
Santino A / 2012 / Hsp90 interaction with Cdc2 and Plo1 kinases contributes to actomyosin ring condensation in fission yeast / Curr Genet 58:191~203
Morrow G / 2015 / Drosophila melanogaster Hsp22: a mitochondrial small heat shock protein influencing the aging process / Front Genet 6:1026
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