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자료유형
학술저널
저자정보
Lee, Ki-Won (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Lee, Sang-Hoon (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Kim, Ki-Yong (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Ji, Hee Chung (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Park, Hyung Soo (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Hwang, Tae Young (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Choi, Gi Jun (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration) Rahman, Md. Atikur (Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration)
저널정보
한국초지조사료학회 한국초지조사료학회지 한국초지조사료학회지 제36권 제3호
발행연도
2016.1
수록면
237 - 242 (6page)

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Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

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