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논문 기본 정보

자료유형
학술저널
저자정보
Joergensen, Mette Warming (Department of Clinical Genetics, Vejle Hospital) Labouriau, Rodrigo (Department of Mathematics, Aarhus University) Hindkjaer, Johnny (The Fertility Clinic and Centre for Preimplantation Genetic diagnosis, Aarhus University Hospital) Stougaard, Magnus (Department of Pathology, Aarhus University Hospital) Kolevraa, Steen (Department of Clinical Genetics, Vejle Hospital) Bolund, Lars (Department of Biomedicine, Aarhus University) Agerholm, Inge Errebo (The Fertility Clinic, Horsens Hospital) Sunde, Lone (Department of Biomedicine, Aarhus University)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제42권 제1호
발행연도
2015.1
수록면
14 - 21 (8page)

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Objective: It has previously been suggested that embryos developing from intracytoplasmic sperm-injected (ICSI) zygotes with three pronuclei (3PN) are endowed with a mechanism for self-correction of triploidy to diploidy. 3PN are also observed in zygotes after conventional in vitro fertilization (IVF). The parental origin, however, differs between the two fertilization methods. Whereas the vast majority of 3PN IVF zygotes are of dispermic origin and thus more likely to have two centrioles, the 3PN ICSI zygotes are digynic in origin and therefore, more likely to have one centriole. In the present study, we examine whether the parental origin of 3PN embryos correlates with the karyotype. Methods: The karyotype of each nucleus was estimated using four sequential fluorescence in situ hybridizations-each with two probes-resulting in quantitative information of 8 different chromosomes. The karyotypes were then compared and correlated to the parental origin. Results: 3PN ICSI embryos displayed a significantly larger and more coordinated reduction from the assumed initial 3 sets of chromosomes than 3PN IVF embryos. Conclusion: The differences in the parental origin-and hence the number of centrioles-between the 3PN IVF and the 3PN ICSI zygotes are likely to be the cause of the differences in karyotypes.

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