지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제38권 제2호
- 발행연도
- 2011.1
- 수록면
- 82 - 86 (5page)
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초록· 키워드
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Objective: To evaluate sperm nuclear DNA fragmentation and chromatin structure after 18 hours' incubation at room temperature. Methods: Twenty-eight male partners who participating IVF treatment were prospectively included in this study. Ejaculated sperm count and motility were assessed. The sperm was then immediately processed by the conventional swim-up method. After utilization of some of the sample for routine clinical use, the remainder of each of the samples was divided into two aliquots. One aliquot was immediately assessed for sperm nuclear DNA fragmentation (TUNEL assay) and chromatin structure (toluidine blue [TB] staining). The other aliquot was incubated at room temperature for 18 hours and then assessed by two methods. Only dark-TB sperms were considered as having abnormal chromatin structure. Data before and after extended incubation were compared using a paired Student's $t$-test. Results: Before and after extended culture, nuclear DNA fragmentation assessed by TUNEL was $4.9{\pm}4.7%$ and $7.0{\pm}6.4%$, respectively ($p$=0.008). The proportion of abnormal chromatin structure (dark-TB sperm) was $8.2{\pm}5.6%$ and $10.3{\pm}6.5%$ ($p$ <0.001), before and after incubation, respectively. Conclusion: After 18 hours' incubation at room temperature, sperm nuclear DNA and chromatin structure were significantly affected. The IVF practitioner should bear this information in mind when performing delayed insemination, especially for $in$ $vitro$ maturation cycles.
목차
등록된 정보가 없습니다.
참고문헌 (31)
참고문헌 신청
Larson KL / 2000 / Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques / Hum Reprod 15:1717~1722
Oosterhuis GJ / 2000 / Measuring apoptosis in human spermatozoa: a biological assay for semen quality / Fertil Steril 74:245~250
Tomlinson MJ / 2001 / Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception / Hum Reprod 16:2160~2165
Morris ID / 2002 / The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development / Hum Reprod 17:990~998
Saleh RA / 2003 / Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility / Fertil Steril 79(3):1597~1605
Oosterhuis GJ / 2000 / Measuring apoptosis in human spermatozoa: a biological assay for semen quality / Fertil Steril 74:245~250
Tomlinson MJ / 2001 / Interrelationships between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception / Hum Reprod 16:2160~2165
Morris ID / 2002 / The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development / Hum Reprod 17:990~998
Saleh RA / 2003 / Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility / Fertil Steril 79(3):1597~1605
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