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논문 기본 정보

자료유형
학술저널
저자정보
Cha, Soo-Kyung (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) Kim, Bo-Yeun (Department of Biomedical Science, College of Life Science, CHA University) Kim, Mi-Kyung (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) Kim, You-Shin (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) Lee, Woo-Sik (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) Yoon, Tae-Ki (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine) Lee, Dong-Ryul (Fertility Center of CHA Gangnam Medical Center, CHA University College of Medicine)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제38권 제1호
발행연도
2011.1
수록면
24 - 30 (7page)

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Objective: The objectives of this study were to analyze efficacy of immature and mature mouse oocytes after vitrification and warming by applying various combinations of cryoprotectants (CPAs) and/or super-rapid cooling using slush nitrogen ($SN_2$). Methods: Four-week old ICR female mice were superovulated for GV- and MII-stage oocytes. Experimental groups were divided into two groups. Ethylene glycol (EG) only group: pre-equilibrated with 1.5 M EG for 2.5 minutes and then equilibrated with 5.5 M EG and 1.0 M sucrose for 20 seconds. EG+dimethylsulfoxide (DMSO) group: pre-equilibrated with 1.3 M EG+1.1 M DMSO for 2.5 minutes and equilibrated with 2.7 M EG+2.1 M DMSO+0.5 M sucrose for 20 seconds. The oocytes were loaded onto grids and plunged into $SN_2$or liquid nitrogen ($LN_2$). Stored oocytes were warmed by a five-step method, and then their survival, maturation, cleavage, and developmental rates were observed. Results: The EG only and EG+DMSO groups showed no significant difference in survival of immature oocytes vitrified after warming. However, maturation and cleavage rates after conventional insemination were greater in the EG only group than in the EG+DMSO group. In mature oocytes, survival, cleavage, and blastocyst formation rates after warming showed no significant difference when EG only or EG+DMSO was applied. Furthermore, cleavage and blastocyst formation rates of MII oocytes vitrified using $SN_2$ were increased in both the EG only and EG+DMSO groups. Conclusion: A combination of CPAs in oocyte cryopreservation could be formulated according to the oocyte stage. In addition, $SN_2$ may improve the efficiency of vitrification by reducing cryoinjury.
#Vitrification #Ethylene Glycol #Dimethyl Sulfoxide #Cooling Rate #Nitrogen #Mice, Inbred ICR #Cryoprotective Agents

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참고문헌 (33)

참고문헌 신청
Chen C / 1986 / Pregnancy after human oocyte cryopreservation / Lancet 1:884~886 google schola van Uem JF / 1987 / Birth after cryopreservation of unfertilized oocytes / Lancet 1:752~753 google schola 이동률 / 2009 / 난자 및 배아동결에 슬러시질소를 이용한 유리화동결의 영향 / Clinical and Experimental Reproductive Medicine 36(1):1~8 dbpia Moller CC / 1989 / Characterization of a proteinase that cleaves zona pellucida glycoprotein ZP2 following activation of mouse eggs / Dev Biol 132:103~112 google schola Mavrides A / 2005 / Bypassing the effect of zona pellucida changes on embryo formation following cryopreservation of bovine oocytes / Eur J Obstet Gynecol Reprod Biol 118:66~70 google schola

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