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논문 기본 정보

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학술저널
저자정보
Kim, Hee-Sun (IVF Laboratory, Department of Obstetrics and Gynecology, Seoul National University Hospital) Kang, Moon Joo (IVF Laboratory, Department of Obstetrics and Gynecology, Seoul National University Hospital) Kim, Sung Ah (IVF Laboratory, Department of Obstetrics and Gynecology, Seoul National University Hospital) Oh, Sun Kyung (IVF Laboratory, Department of Obstetrics and Gynecology, Seoul National University Hospital) Kim, Hoon (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) Ku, Seung-Yup (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) Kim, Seok Hyun (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) Moon, Shin Yong (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) Choi, Young Min (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제40권 제1호
발행연도
2013.1
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23 - 28 (6page)

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Objective: The aim of the present study was to examine the relationship among male age, strict morphology, and sperm chromatin structure and condensation. Methods: Sperm samples from a total of 100 men underwent semen analysis, and sperm chromatin structure and condensation were assessed with toluidine blue (TB) and aniline blue (AB) tests. Results: Prevalence of strict morphology of less than 4%, and abnormal sperm chromatin structure and condensation did not show any statistically significant differences according to male age (p=0.605, p=0.235, and p=0.080). No significant correlation was demonstrated among age of male partners, strict morphology, and abnormal sperm chromatin structure using TB and AB tests. However, abnormal sperm chromatin condensation was positively associated with sperm chromatin structure (r=0.594, p=0.000) and showed negative correlation with strict morphology (r=-0.219, p=0.029). Conclusion: The tests for sperm chromatin condensation showed a significant association with strict morphology. Further study is needed to elucidate the relationship between clinical outcome and sperm chromatin tests.
#Toluidine blue #Aniline blue #Semen analysis #DNA damage #Human

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참고문헌 (23)

참고문헌 신청
World Health Organization / WHO laboratory manual for the examination and processing of human semen google schola Guzick DS / 2001 / Sperm morphology, motility, and concentration in fertile and infertile men / N Engl J Med 345:1388~1393 google schola Evenson DP / 1999 / Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic / Hum Reprod 14:1039~1049 google schola Carrell DT / 2003 / Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss / Arch Androl 49:49~55 google schola Virro MR / 2004 / Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles / Fertil Steril 81:1289~1295 google schola

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