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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 670 - 680 (11page)
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Acanthophysium sp. KMF001, a wood rotting fungus, produces a strong crude enzyme complex that efficiently produces simple sugars from wood. The transcriptomic analysis of Acanthophysium sp. KMF001 identified 14 genes for putative glycoside hydrolases. Among them, isotig01043 was expressed heterogeneously in Escherichia coli BL21(DE3), and the expressed protein exhibited an endo-${\beta}$-1,4-xylanase activity which showed the optimum reaction at pH 5.0 and $30^{\circ}C$. The enzyme kinetic values of $K_m$ and $V_{max}$ were 25.92 mg/ml and $0.628{\mu}mole/mg/ml$, respectively. The enzymatic characteristics of the expressed xylanase showed a typical fungal xylanase. However, the bioinformatics analysis suggested that the protein encoded by isotig01043 was a novel xylanase based on a low identity when it was compared with the closest protein in the NCBI database and a similar protein domain with GH16_fungal_Lam16A_glucanase, which had not been earlier suggested as a xylanase.
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참고문헌 (27)
참고문헌 신청
Beg, Q. / 2001 / Microbial xylanases and their industrial applications: a review / Applied Microbiology and Biotechnology 56(3):326~338
Bradford, M.M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Analytical Biochemistry 72(1):248~254
Chedgy, R.J. / 2009 / Effects of leaching on fungal growth and decay of western red cedar / Canadian Journal of Microbiology 55(5):578~586
Guo, B. / 2009 / Gene cloning, expression and characterization of a new cold-active and salttolerant endo-β-1,4-xylanase from marine Glaciecola mesophila KMM 241 / Applied Microbiology and Biotechnology 84(6):1107~1115
Joo, A.R. / 2009 / Purification and characterization of a β-1,4-glucosidase from a newly isolated strain of Fomitopsis pinicola / Applied Microbiology and Biotechnology 83(2):285~294
Bradford, M.M. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Analytical Biochemistry 72(1):248~254
Chedgy, R.J. / 2009 / Effects of leaching on fungal growth and decay of western red cedar / Canadian Journal of Microbiology 55(5):578~586
Guo, B. / 2009 / Gene cloning, expression and characterization of a new cold-active and salttolerant endo-β-1,4-xylanase from marine Glaciecola mesophila KMM 241 / Applied Microbiology and Biotechnology 84(6):1107~1115
Joo, A.R. / 2009 / Purification and characterization of a β-1,4-glucosidase from a newly isolated strain of Fomitopsis pinicola / Applied Microbiology and Biotechnology 83(2):285~294
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