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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 76 - 81 (6page)
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초록· 키워드
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The protease produced by a Bacillus pumilus CN8 strain was purified by DEAE-Cellulose-52 ion exchange. It has a molecular weight of approximately 96,920 Dalton. In the present study, this protease showed strong activity over a broad range of pH (6.5-9.5) and temperature from $40^{\circ}C$ to $60^{\circ}C$, and the protease performed the maximal activity at pH 7.3 at $42^{\circ}C$. The effect of metal ions on protease activity showed that $K^+$ could slightly increase the protease activity, and other ions such as $Zn^{2+}$, $Fe^{2+}$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$ had no significant activation or inhibition to the protease (P> 0.05), and the more important is that $Cu^{2+}$, $Mn^{2+}$, $Sn^{2+}$, $Cd^{2+}$ had a strong inhibitory effect on the protease activity.
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참고문헌 (23)
참고문헌 신청
Ozturk, D.C. / 2010 / Water miscible mono alcohols effect on the structural conformation of Bacillus clausii GMBAE 42 serine alkaline protease / J. Mol. Catal. B: Enzym 64:184~188
Johnvesly, B. / 2001 / Studies on production of thermostable alkaline protease from thermophilic and alkaliphilic Bacillus sp. JB-99 in a chemically defined medium / Process Biochem 37:139~144
Bhaskar, N. / 2007 / Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities / Bioresour. Technol 98:2758~2764
Sareen, R. / 2008 / Purification and characterization of organic solvent stable protease from Bacillus licheniformis RSP-09-37 / Appl. Microbiol. Biotechnol 79:399~405
Jellouli, K. / 2009 / Molecular and biochemical characterization of an extracellular serine-protease from Vibrio metschnikovii J1 / J. Ind. Microbiol. Biotechnol 36:939~948
Johnvesly, B. / 2001 / Studies on production of thermostable alkaline protease from thermophilic and alkaliphilic Bacillus sp. JB-99 in a chemically defined medium / Process Biochem 37:139~144
Bhaskar, N. / 2007 / Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities / Bioresour. Technol 98:2758~2764
Sareen, R. / 2008 / Purification and characterization of organic solvent stable protease from Bacillus licheniformis RSP-09-37 / Appl. Microbiol. Biotechnol 79:399~405
Jellouli, K. / 2009 / Molecular and biochemical characterization of an extracellular serine-protease from Vibrio metschnikovii J1 / J. Ind. Microbiol. Biotechnol 36:939~948
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