Inhibition of Leptin and Leptin Receptor Gene Expression by Silibinin-Curcumin Combination

Nejati-Koshki, Kazem; Akbarzadeh, Abolfazl; Pourhasan-Moghaddam, Mohammad; Abhari, Alireza; Dariushnejad, Hassan

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자료유형: 학술저널

저자정보: Nejati-Koshki, Kazem (Department of Medical Biotechnology, Faculty of Medicine, Tabriz University of Medical Sciences) Akbarzadeh, Abolfazl (Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences) Pourhasan-Moghaddam, Mohammad (Department of Medical Biotechnology, Faculty of Medicine, Tabriz University of Medical Sciences) Abhari, Alireza (Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences) Dariushnejad, Hassan (Department of Medical Biotechnology, Faculty of Medicine, Tabriz University of Medical Sciences)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제11호

발행연도: 2013.1

수록면: 6,595 - 6,599 (5page)

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Leptin and its receptor are involved in breast carcinogenesis as mitogenic factors. Therefore, they could be considered as targets for breast cancer therapy. Expression of the leptin receptor gene could be modulated by leptin secretion. Silibinin and curcumin are herbal compounds with anti-cancer activity against breast cancer. The aim of this study was to assess their potential to inhibit of expression of the leptin gene and its receptor and leptin secretion. Cytotoxic effects of the two agents on combination on T47D breast cancer cells was investigated by MTT assay test after 24h treatment. With different concentrations the levels of leptin, leptin receptor genes expression were measured by reverse-transcription real-time PCR. Amount of secreted leptin in the culture medium was determined by ELISA. Data were statistically analyzed by one-way ANOVA test. The silibinin and curcumin combination inhibited growth of T47D cells in a dose dependent manner. There were also significant difference between control and treated cells in leptin expression and the quantity of secreted leptin with a relative decrease in leptin receptor expression. In conclusion, these herbal compounds inhibit the expression and secretion of leptin and it could probably be used as drug candidates for breast cancer therapy through leptin targeting in the future.

#Leptin #silibinin #curcumin #breast cancer #T47D cell line

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Nejati-Koshki, Kazem

소속기관 Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Zanjan University of Me