Prevalence of Aflatoxin Induced p53 Mutation at Codon 249 (R249s) in Hepatocellular Carcinoma Patients with and without Hepatitis B Surface Antigen (HBsAg)

Chittmittrapap, Salyavit; Chieochansin, Thaweesak; Chaiteerakij, Roongruedee; Treeprasertsuk, Sombat; Klaikaew, Naruemon; Tangkijvanich, Pisit; Komolmit, Piyawat; Poovorawan, Yong

추천

검색

자료유형: 학술저널

저자정보: Chittmittrapap, Salyavit (Liver Disease and Liver Cancer Research Unit, Department of Biochemistry, Chulalongkorn University) Chieochansin, Thaweesak (Center of Excellence in Clinical Virology, Department of Pediatrics, Chulalongkorn University) Chaiteerakij, Roongruedee (Department of Internal Medicine, Chulalongkorn University) Treeprasertsuk, Sombat (Department of Internal Medicine, Chulalongkorn University) Klaikaew, Naruemon (Department of Pathology, Chulalongkorn University) Tangkijvanich, Pisit (Liver Disease and Liver Cancer Research Unit, Department of Biochemistry, Chulalongkorn University) Komolmit, Piyawat (Department of Internal Medicine, Chulalongkorn University) Poovorawan, Yong (Center of Excellence in Clinical Virology, Department of Pediatrics, Chulalongkorn University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제14권 제12호

발행연도: 2013.1

수록면: 7,675 - 7,679 (5page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: A missense mutation in exon 7 (R249S) of the p53 tumor suppressor gene is characteristic of aflatoxin B1 (AFB1) exposure. AFB1 is believed to have a synergistic effect on hepatitis virus B (HBV) carcinogenesis. However, results of studies comparing R249S prevalence among patients are conflicting. The aim of this study was to determine the prevalence of the R249S mutation in hepatocellular carcinoma (HCC) patients with or without positive HBsAg. Materials and Methods: Paraffin embedded liver tissues were obtained from 124 HCC patients who underwent liver resection and liver biopsy in King Chulalongkorn Memorial Hospital. Restriction fragment length polymorphism (RFLP) was utilized to detect the R249S mutation. Positive results were confirmed by direct sequencing. Results: Sixty four (52%) patients were positive for HBsAg and 18 (15%) were anti-HCV positive. 12 specimens tested positive by RFLP. Ten HCC patients (8.1%) were confirmed to be R249S positive by Sanger sequencing (AGG to AGT). Out of these 10, six were HBsAg positive, and out of the remaining 4, two were anti-HCV positive. The R249S prevalence among HCC patients with positive HBsAg was 9.4% compared to 6.7% for HBsAg negative samples. Patients with the R249S mutation were younger ($55{\pm}10$ vs $60{\pm}13$ year-old) and tended to have a more advanced Edmonson-Steiner grade of HCC, although differences did not reach statistical significance. Conclusions: Our study shows moderate prevalence of aflatoxin B1-related p53 mutation (R249S) in HCC with or without HBsAg. HBsAg positive status was not associated with R249S prevalence.

#Aflatoxin B1 #R249S #p53 mutation #249ser #Hepatocellular carcinoma #HBsAg

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

이 논문의 저자 정보

Chittmittrapap, Salyavit

소속기관 Liver Disease and Liver Cancer Research Unit, Department of Biochemistry, Chulalongkorn University