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논문 기본 정보

자료유형
학술저널
저자정보
Dang, Ya-Mei (First Clinical Medical College of Lanzhou University) Huang, Gang (Gansu Provincial Hospital) Chen, Yi-Rong (First Clinical Medical College of Lanzhou University) Dang, Zhong-Feng (The Tumor Hospital of Gansu Province) Chen, Cheng (Gansu Provincial Hospital) Liu, Feng-Lei (Gansu Provincial Hospital) Guo, Ying-Fang (Gansu Provincial Hospital) Xie, Xiao-Dong (School of Basic Medical Sciences, Lanzhou University)
저널정보
아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제4호
발행연도
2014.1
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1,517 - 1,520 (4page)

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Aim: To investigate effects of sulforaphane on the BIU87 cell line and underlying mechanisms involving IGFBP-3. Methods: Both BIU87 and IGFBP-3-silenced BIU87 cells were treated with sulforaphane. Cell proliferation was detected by MTT assay. Cell cycle and apoptosis were determined via flow cytometry. Quantitative polymerase chain reaction and Western blotting were applied to analyze the expression of IGFBP-3 and NF-${\kappa}B$ at both mRNA and protein levels. Results: Sulforaphane (80 ${\mu}M$) treatment could inhibit cell proliferation, inducing apoptosis and cell cycle arrest at G2/M phase. All these effects could be antagonized by IGFBP-3 silencing. Furthermore, sulforaphane (80 ${\mu}M$) could down-regulate NF-${\kappa}B$ expression while elevating that of IGFBP-3. Conclusions: Sulforaphane could suppress the proliferation of BIU87 cells via enhancing IGFBP-3 expression, which negatively regulating the NF-${\kappa}B$ signaling pathway.

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