Ginsenoside Rg1 Induces Apoptosis through Inhibition of the EpoR-Mediated JAK2/STAT5 Signalling Pathway in the TF-1/Epo Human Leukemia Cell Line

Li, Jing; Wei, Qiang; Zuo, Guo-Wei; Xia, Jing; You, Zhi-Mei; Li, Chun-Li; Chen, Di-Long

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자료유형: 학술저널

저자정보: Li, Jing (Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University) Wei, Qiang (Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University) Zuo, Guo-Wei (Key Laboratories of Clinical Diagnostics, Province and Ministry of Education, Chongqing Medical University) Xia, Jing (Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University) You, Zhi-Mei (Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University) Li, Chun-Li (Institute of Life Sciences, Chongqing Medical University) Chen, Di-Long (Laboratory of Stem Cells and Tissue Engineering, Department of Histology and Embryology, Chongqing Medical University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제15권 제6호

발행연도: 2014.1

수록면: 2,453 - 2,459 (7page)

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Ginsenoside Rg1 is one effective anticancer and antioxidant constituent of total saponins of Panax ginseng (TSPG), which has been shown to have various pharmacological effects. Our previous study demonstrated that Rg1 had anti-tumor activity in K562 leukemia cells. The aim of this study was designed to investigate whether Rg1 could induce apoptosis in TF-1/Epo cells and further to explore the underlying molecular mechanisms. Here we found that Rg1 could inhibit TF-1/Epo cell proliferation and induce cell apoptosis in vitro in a concentration and time dependent manner. It also suppressed the expression of EpoR on the surface membrane and inhibited JAK2/STAT5 pathway activity. Rg1 induced up-regulation of Bax, cleaved caspase-3 and C-PAPR protein and down-regulation of Bcl-2 and AG490, a JAK2 specific inhibitor, could enhance the effects of Rg1. Our studies showed that EpoR-mediated JAK2/STAT5 signaling played a key role in Rg1-induced apoptosis in TF-1/Epo cells. These results may provide new insights of Rg1 protective roles in the prevention a nd treatment of leukemia.

#Ginsenoside Rg1 #apoptosis #erythropoietin receptor #JAK2 #STAT5 #leukemia

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Li, Jing

소속기관 Department of Gynecology Oncology, the Affiliated Tumor Hospital of Guangxi Medical University