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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Lymphocystis is a viral disease of fish primarily in marine and brackishwaters. Here we report the cloning, expression, and the serological applications of the lymphocystis disease virus (LCDV) major capsid protein (MCP). The MCP gene was amplified by PCR from the genomic DNA of LCDV isolated from Schlegel's black rockfish, Sebastes schlegeli, and expressed in E. coli. Mouse antisera raised against the purified recombinant MCP (rMCP) reacted with the viral MCP in an immunofluorescence assay, indicating that this rMCP would be useful for serological studies of field samples.
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참고문헌 (14)
참고문헌 신청
Ausubel, F.M. / 2002 / Short protocols in molecular biology / Wiley
Cano, I. / 2007 / Development of molecular techniques for detection of lymphocystis disease virus in different marine fish species / J. Appl. Microbiol 102:32~40
Cheng, S. / 2006 / Development and characterization of monoclonal antibody to the lymphocystis disease virus of Japanese flounder Paralichthys olivaceus isolated from China / J. Virol. Methods 135:173~180
Dixon, P. / 1996 / Preliminary study of the detection of antibodies to lymphocystis disease virus in flounder, Platichthys flesusL., exposed to contaminated harbour sludge / Fish Shellfish Immunol 6:123~133
Hu, G.B. / 2004 / Induction of apoptosis in a flounder gill cell line by lymphocystis disease virus infection / J. Fish Dis 27:657~662
Cano, I. / 2007 / Development of molecular techniques for detection of lymphocystis disease virus in different marine fish species / J. Appl. Microbiol 102:32~40
Cheng, S. / 2006 / Development and characterization of monoclonal antibody to the lymphocystis disease virus of Japanese flounder Paralichthys olivaceus isolated from China / J. Virol. Methods 135:173~180
Dixon, P. / 1996 / Preliminary study of the detection of antibodies to lymphocystis disease virus in flounder, Platichthys flesusL., exposed to contaminated harbour sludge / Fish Shellfish Immunol 6:123~133
Hu, G.B. / 2004 / Induction of apoptosis in a flounder gill cell line by lymphocystis disease virus infection / J. Fish Dis 27:657~662
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