지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국광과학회 Photochemical & photobiological sciences : an international journal Photochemical & photobiological sciences : an international journal 제8권 제10호
- 발행연도
- 2009.1
- 수록면
- 1,461 - 1,466 (6page)
이용수
초록· 키워드
오류제보하기
Synthesis of protoporphyrin IX (PpIX) by malignant cells is essential for the success of ALA-based photodynamic therapy (PDT). Two key enzymes that were described as affecting PpIX accumulation during ALA treatment are porphobilinogen deaminase (PBGD) and ferrochelatase. Here, we show that down regulation of ALA dehydratase (ALAD) expression and activity by specific shRNA induced a marked decrease in PpIX synthesis in K562 erythroleukemic cells. Photo-inactivation efficacy following ALA-PDT was directly correlated with ALAD-silencing and cellular levels of PpIX. MTT metabolism following ALA-PDT was shown to be 60% higher in ALAD-silenced cells in comparison to control cells, indicating that mitochondria were protected in the silenced cells. Morphological analysis by scanning electron microscopy (SEM) of cells treated by ALA-PDT showed no morphological changes in ALAD-silenced cells, in contrast to controls exhibiting cell deformations and lysis. Membrane integrity following ALA-PDT was kept intact and undamaged in ALAD-silenced cells as examined by Annexin V-FITC/PI staining and LDH-L leakage. We conclude that ALAD, although it is present in the cell at abundant levels, has a major and limiting role in regulating PpIX synthesis and ALA-PDT outcome.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
The Effects of ALA-PDT on Leukemia Cells and Hepatoma Cells
Journal of photoscience : an international journal officail organ of the korean society of photoscience
2002 .01
-
포유류 세포주에서 δ-Aminolevulinic acid (ALA)의 세포증식과 사멸에 미치는 영향분석
분석과학
2014 .10
-
Apoptotic Signaling Cascade of 5-aminolaevulinic Acid-based Photodynamic Therapy in Human Promyelocytic Leukemia HL-60 Cells
Journal of photoscience : an international journal officail organ of the korean society of photoscience
2002 .01
-
Rhodobacter capsulatus hemA 유전자 발현 대장균에 의한 5-Aminolevulinic Acid 생산의 최적화
한국미생물·생명공학회지
2009 .01
-
5-Aminolevulinic Acid Biosynthesis in Escherichia coli CoexpressingNADP-dependent Malic Enzyme and 5-Aminolevulinate Synthase
Journal of Microbiology and Biotechnology
2007 .01
-
Protective effects of perilla oil and alpha linolenic acid on SH-SY5Y neuronal cell death induced by hydrogen peroxide
Nutrition Research and Practice
2018 .04
-
Protective effects of perilla oil and alpha linolenic acid on SH-SY5Y neuronal cell death induced by hydrogen peroxide
Nutrition Research and Practice
2018 .04
-
Bacillus cereus 1-1 균주의 5-Aminolevulinic Acid (ALA) 생산
미생물학회지
2007 .12
-
농촌 여성들의 ALAD 유전형질별 철분영양상태와 철분제 섭취에 따른 영양상태의 변화
대한지역사회영양학회지
2006 .12
-
Extracellular 5-Aminolevulinic Acid Production by Escherichia coli Containingthe Rhodopseudomonas palustris KUGB306 hemA Gene
Journal of Microbiology and Biotechnology
2008 .01
-
Topical photodynamic therapy with porphyrin precursors.assessment of treatment-associated pain in a retrospective study
Photochemical & photobiological sciences : an international journal
2009 .01
-
In Vitro expression of protoporphyrin IX induced by 5-aminolevulinic acid (ALA) in human cancer and normal cells
한국생명과학회 심포지움
2006 .09
-
Optimization of Influencing Factors on Biomass Accumulation and 5-Aminolevulinic Acid (ALA) Yield in Rhodobacter sphaeroides Wastewater Treatment
Journal of Microbiology and Biotechnology
2015 .01
-
Fabrication of an α-lipoic acid-eluting poly-(D,L-lactide-cocaprolactone) cuff for the inhibition of neointimal formation
Experimental and Molecular Medicine
2009 .01
-
Process Strategies to Enhance the Production of 5-Aminolevulinic Acid with Recombinant E. coli
Journal of Microbiology and Biotechnology
2004 .01
-
Successful treatment of topical photodynamic therapy using 5-aminolevulinic acid for lymphadenosis benigna cutis
Journal of photoscience : an international journal officail organ of the korean society of photoscience
2002 .01
-
(InAs)n(AlAs)n 단주기 초결자의 MBE 성장과 X선회절
Applied Science and Convergence Technology
1992 .10
-
Photodynamic Therapy-Mediated Temporal Expression of Thymidine Kinase Genes Ligated to the Human Heat Shock Promotor: Preliminary in vitro Model Study of Enhanced Phototoxicity by PDT-Induced Gene Therapy
Journal of photoscience : an international journal officail organ of the korean society of photoscience
2002 .01
-
Bandgap Engineering of self - assembled InAs quantum dots with a thin AlAs barrier
한국진공학회 학술발표회초록집
2003 .08
-
The role of p53 in the efficiency of photodynamic therapy with hypericin and subsequent long-term survival of colon cancer cells
Photochemical & photobiological sciences : an international journal
2009 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0