지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
To determine whether rock bream Oplegnathus fasciatus can be protected from rock bream iridovirus (RBIV) infection by intramuscular injection of long double-stranded RNAs (dsRNAs), we compared protective effect of virus-specific dsRNAs corresponding to major capsid protein (MCP), ORF 084, ORF 086 genes, and virus non-specific green fluorescent protein (GFP) gene. Furthermore, to determine whether the non-specific type I interferon (IFN) response was associated with protective effect, we estimated the activation of type I IFN response in fish using expression level of IFN inducible Mx gene as a marker. As a result, mortality of fish injected with dsRNAs and challenged with RBIV was delayed for a few days when comparing with PBS injected control group. However, virus-specific dsRNA injected groups exhibited no significant differences in survival period when compared to the GFP dsRNA injected group. Semi-quantitative analysis indicated that the degree of antiviral response via type I IFN response is supposedly equal among dsRNA injected fish. These results suggest that type I IFN response rather than sequence-specific RNA interference might involve in the lengthened survival period of fish injected with virus-specific dsRNAs.
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등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
Bagasra, O. / 2005 / RNAi as an antiviral therapy / Expert. Opin. Biol. Ther. 5:1463~1474
Bumcrot, D. / 2006 / RNAi therapeutics: a potential new class of pharmaceutical drugs / Nat. Chem. Biol. 2:711~719
Caipang, C.M. / 2003 / In vitro inhibition of fish rhabdoviruses by Japanese flounder, Paralichthys olivaceus Mx / Virology 317:373~382
Caipang, C.M. / 2006 / Genetic vaccines protect red seabream, Pagrus major, upon challenge with red seabream iridovirus (RSIV) / Fish Shellfish Immunol. 21:130~138
de Veer, M.J. / 2001 / Functional classification of interferon-stimulated genes identified using microarrays / J. Leukoc. Biol. 69:912~920
Bumcrot, D. / 2006 / RNAi therapeutics: a potential new class of pharmaceutical drugs / Nat. Chem. Biol. 2:711~719
Caipang, C.M. / 2003 / In vitro inhibition of fish rhabdoviruses by Japanese flounder, Paralichthys olivaceus Mx / Virology 317:373~382
Caipang, C.M. / 2006 / Genetic vaccines protect red seabream, Pagrus major, upon challenge with red seabream iridovirus (RSIV) / Fish Shellfish Immunol. 21:130~138
de Veer, M.J. / 2001 / Functional classification of interferon-stimulated genes identified using microarrays / J. Leukoc. Biol. 69:912~920
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