지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Polyporus umbellatus (Syn. Grifola umbellata) is a sclerotium forming mushroom belongs to family Polyporaceae of Polyphorales, Basidiomycota. The sclerotia of P. umbellatus have long been used for traditional medicines in China, Korea and Japan. This study was initiated to obtain the basic data for artificial sclerotial production of P. umbellatus. Here, we investigated the favorable conditions for mycelial growth of P. umbellatus and its symbiotic fungus Armillaria mellea. We also evaluate the favorable carbon and nitrogen sources for sclerotial formation in dual culture between P. umbellatus and A. mellea. The favorable conditions for mycelial growth of P. umbellatus were $20^{\circ}C$ and pH 4, while optimal conditions for mycelial growth of A. mellea were $25^{\circ}C$ and pH 6. The carbon sources for optimal mycelial growth of P. umbellatus were fructose and glucose, while carbon sources for favorable mycelial growth of A. mellea were also fructose and glucose. The nitrogen sources for favorable mycelial growth P. umbellatus were peptone and yeast extract, while optimal mycelial growth of A. mellea were obtained in peptone and yeast extract. When P. umbellatus and A. mellea were dual cultured on carbon sources, sclerotia were induced on basal media supplemented with glucose, fructose and maltose at pH 4~6, while nitrogen sources inducing sclerotia were basal media supplemented with peptone and yeast extract for 60 days at $20^{\circ}C$ under dark condition.
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참고문헌 (16)
참고문헌 신청
Cheng, X. H / 2006 / Factors influencing formation of sclerotia in Grifola umbellate (Pers.) Pilt under artificial conditions / J Integr Plant Biol 48(11):1312~1317
Liu, Y. Y. / 2009 / Nutritional fators determining sclerotial formation of Polyporus umbellatus / Lett Appl Microbiol 49(2):283~288
Guo, S. X / 1991 / Nutrient source of sclerotia of Grifola umbellata and its relationship to Armillaria mellea / Acta Bot Sin 34:576~580
Kikuchi, G / 2010 / Identificantion of Armillaria species associated with Polyporus umbellatus using ITS sequences of nuclear ribosomal DNA / Mycoscience 51:366~372
Lu, W / 1985 / Platelet aggregation potentiators from Cho-Rei. Chem / Pharm. Bull 33(1):5083~5087
Liu, Y. Y. / 2009 / Nutritional fators determining sclerotial formation of Polyporus umbellatus / Lett Appl Microbiol 49(2):283~288
Guo, S. X / 1991 / Nutrient source of sclerotia of Grifola umbellata and its relationship to Armillaria mellea / Acta Bot Sin 34:576~580
Kikuchi, G / 2010 / Identificantion of Armillaria species associated with Polyporus umbellatus using ITS sequences of nuclear ribosomal DNA / Mycoscience 51:366~372
Lu, W / 1985 / Platelet aggregation potentiators from Cho-Rei. Chem / Pharm. Bull 33(1):5083~5087
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