지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
In the bipolar basidiomycete Pholiota nameko, a pair of homeodomain protein genes located at the A mating-type locus regulates mating compatibility. In the present study, we used a DNA-mediated transformation system in P. nameko to investigate the homeodomain proteins that control the clamp formation. When a single homeodomain protein gene (A3-hox1 or A3-hox2) from the A3 monokaryon strain was introduced into the A4 monokaryon strain, the transformants produced many pseudo-clamps but very few clamps. When two homeodomain protein genes (A3-hox1 and A3-hox2) were transformed either separately or together into the A4 monokaryon, the ratio of clamps to the clamp-like cells in the transformants was significantly increased to approximately 50%. We, therefore, concluded that the gene dosage of homeodomain protein genes is important for clamp formation. When the sip promoter was connected to the coding region of A3-hox1 and A3-hox2 and the fused fragments were introduced into NGW19-6 (A4), the transformants achieved more than 85% clamp formation and exhibited two nuclei per cell, similar to the dikaryon (NGW12-163 ${\times}$ NGW19-6). The results of real-time RT-PCR confirmed that sip promoter activity is greater than that of the native promoter of homeodomain protein genes in P. nameko. So, we concluded that nearly 100% clamp formation requires high expression levels of homeodomain protein genes and that altered expression of the A mating-type genes alone is sufficient to drive true clamp formation.
목차
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참고문헌 (33)
참고문헌 신청
Aimi, T. / 2005 / Identification and linkage mapping of the genes for the putative homeodomain protein (hox1) and the putative pheromone receptor protein homologue (rcb1) in a bipolar basidiomycete Pholiota nameko / Curr Genet 48:184~194
Bakkeren, G / 1994 / Linkage of mating-type loci distinguishes bipolar from tetrapolar mating in basidiomycetous smut fungi / Proc.Natl.Acad. Sci.USA 92:7085~7089
Banham, A. H. / 1995 / An N-terminal dimerization domain permits homeodomain proteins to choose compatible partners and initiate sexual development in the mushroom Coprinus cinereus / Plant Cell 7:773~783
Birnboim, H. C / 1983 / A rapid alkaline extraction method for the isolation of plasmid DNA 100:243~255
Casselton, L. A / 2006 / The mating type genes of the basidiomycetes,iIn The Mycota Growth, Differentiation and Sexuality, 2nd edition, vol. 1, part. 17 / Springer :357~374
Bakkeren, G / 1994 / Linkage of mating-type loci distinguishes bipolar from tetrapolar mating in basidiomycetous smut fungi / Proc.Natl.Acad. Sci.USA 92:7085~7089
Banham, A. H. / 1995 / An N-terminal dimerization domain permits homeodomain proteins to choose compatible partners and initiate sexual development in the mushroom Coprinus cinereus / Plant Cell 7:773~783
Birnboim, H. C / 1983 / A rapid alkaline extraction method for the isolation of plasmid DNA 100:243~255
Casselton, L. A / 2006 / The mating type genes of the basidiomycetes,iIn The Mycota Growth, Differentiation and Sexuality, 2nd edition, vol. 1, part. 17 / Springer :357~374
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