메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
Wan, Jianing (Faculty of Agriculture, Tottori University) Yi, Ruirong (Faculty of Agriculture, Tottori University) Li, Yan (Faculty of Agriculture, Tottori University) Kinjo, Yukiko (Faculty of Agriculture, Tottori University) Sadashima, Aki (Faculty of Agriculture, Tottori University) Terashita, Takao (Faculty of Agriculture, Kinki University) Yamanaka, Katsuji (Kyoto Mycological Institute) Aimi, Tadanori (Faculty of Agriculture, Tottori University)
저널정보
한국버섯학회 한국버섯학회지 한국버섯학회지 제9권 제2호
발행연도
2011.1
수록면
53 - 58 (6page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
In order to confirm the presence of putative glucoamylase gene in Tricholoma matsutake genome, the genomic DNA was prepared from T. matsutake NBRC30773 strain and was used as template to clone the glucoamylases gene (TmGlu1). We obtained the nucleotide sequence of TmGlu1 and its franking region. The coding region (from ATG to stop codon) is 2,186 bp. The locations of exons and introns were determined from the nucleotide sequences of 3'- and 5'-RACE PCR and RT-PCR products. On the other hand, to investigate the relationship between composition of medium and glucoamylase expression, we checked the expression level of glucoamylase gene by realtime reverse transcription PCR and measurement of glucoamylase enzyme activity. It was found that enzyme activity of glucoamylase was very low in different medium. Expression of glucoamylases gene appeared to not be affected by different carbon source.

목차

등록된 정보가 없습니다.

참고문헌 (12)

참고문헌 신청

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0