지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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In order to confirm the presence of putative glucoamylase gene in Tricholoma matsutake genome, the genomic DNA was prepared from T. matsutake NBRC30773 strain and was used as template to clone the glucoamylases gene (TmGlu1). We obtained the nucleotide sequence of TmGlu1 and its franking region. The coding region (from ATG to stop codon) is 2,186 bp. The locations of exons and introns were determined from the nucleotide sequences of 3'- and 5'-RACE PCR and RT-PCR products. On the other hand, to investigate the relationship between composition of medium and glucoamylase expression, we checked the expression level of glucoamylase gene by realtime reverse transcription PCR and measurement of glucoamylase enzyme activity. It was found that enzyme activity of glucoamylase was very low in different medium. Expression of glucoamylases gene appeared to not be affected by different carbon source.
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참고문헌 (12)
참고문헌 신청
Birnboim, H. C / 1983 / A rapid alkaline extraction method for the isolation of plasmid DNA / Methods Enzymol 100:243~255
Fowler, T / 1990 / Regulation of the glaA gene of Aspergillus niger / Curr Genet 18:537~545
Hirokawa, T. / 1998 / SOSUI: classification and secondary structure prediction system for membrane proteins / Bioinformatics 14:378~379
Horton, P / 1997 / Better prediction of protein cellular localization sites with the knearest neighbors classifier / Proc Int Conf Intell Syst Mol Bio 15:147~152
Martin, F. / 2008 / The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis / Nature 452:88~92
Fowler, T / 1990 / Regulation of the glaA gene of Aspergillus niger / Curr Genet 18:537~545
Hirokawa, T. / 1998 / SOSUI: classification and secondary structure prediction system for membrane proteins / Bioinformatics 14:378~379
Horton, P / 1997 / Better prediction of protein cellular localization sites with the knearest neighbors classifier / Proc Int Conf Intell Syst Mol Bio 15:147~152
Martin, F. / 2008 / The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis / Nature 452:88~92
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