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Mesocosm experiments conducted for ecological purposes have become increasingly popular because they can provide a holistic understanding of the biological complexities associated with natural systems. This paper describes a new outdoor mesocosm designed for $CO_2$ perturbation experiments of benthos. Manipulated the carbonate chemistry in a continuous flow-through system can be parallelized with diurnal changes, while irradiance, temperature, and nutrients can vary according to the local environment. A target hydrogen ion activity (pH) of seawater was sufficiently stabilized and maintained within 4 h after dilution, which was initiated by the ratio of $CO_2$-saturated seawater to ambient seawater. Specifically, pH and $CO_2$ partial pressure ($pCO_2$) levels gradually varied from 8.05-7.28 and $375-2,691{\mu}atm$, respectively, over a range of dilution ratios. This mesocosm can successfully manipulate the pH and $pCO_2$ of seawater, and it demonstrates suitability for ocean acidification experiments on benthic communities.
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참고문헌 (38)
참고문헌 신청
Alexandre, A. / 2012 / Effects of CO2 enrichment on photosynthesis, growth, and nitrogen metabolism of the seagrass Zostera noltii / Ecol Evol 2:2625~2635
Andersson, AJ. / 2009 / Net loss of CaCO3 from a subtropical calcifying community due to seawater acidification: mesocosm-scale experimental evidence / Biogeosciences 6:1811~1823
Barry, JP. / 2010 / Guide for Best Practices in Ocean Acidification Research and Data Reporting / Publications Office of the European Union :123~136
Burrell, RB. / 2015 / An automated monitoring and control system for flow-through co-cycling hypoxia and pH experiments / Limnol Oceanogr Methods 14:168~185
Campbell, JE. / 2011 / Novel methodology for in situ carbon dioxide enrichment of benthic ecosystems / Limnol. Oceanogr. Methods 9:97~109
Andersson, AJ. / 2009 / Net loss of CaCO3 from a subtropical calcifying community due to seawater acidification: mesocosm-scale experimental evidence / Biogeosciences 6:1811~1823
Barry, JP. / 2010 / Guide for Best Practices in Ocean Acidification Research and Data Reporting / Publications Office of the European Union :123~136
Burrell, RB. / 2015 / An automated monitoring and control system for flow-through co-cycling hypoxia and pH experiments / Limnol Oceanogr Methods 14:168~185
Campbell, JE. / 2011 / Novel methodology for in situ carbon dioxide enrichment of benthic ecosystems / Limnol. Oceanogr. Methods 9:97~109
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