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논문 기본 정보

자료유형
학술저널
저자정보
Park, Jaeyeon (Environment and Resource Convergence Center, Advanced Institutes of Convergence Technology) Jeong, Hae Jin (Environment and Resource Convergence Center, Advanced Institutes of Convergence Technology) Yoon, Eun Young (Environment and Resource Convergence Center, Advanced Institutes of Convergence Technology) Moon, Seung Joo (Environment and Resource Convergence Center, Advanced Institutes of Convergence Technology)
저널정보
한국조류학회(藻類) Algae Algae 제31권 제4호
발행연도
2016.1
수록면
391 - 401 (11page)

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To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only $1-3{\times}10^5cells$ of P. minimum and A. minutum, $10^4cells$ of P. micans, and $10^3cells$ of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.

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