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논문 기본 정보

자료유형
학술저널
저자정보
Kim, In-Cheul (National Institute of Animal Science, RDA) Han, Deug-Woo (Noah Biotech Inc.) Lee, Sung-Won (Noah Biotech Inc.) Ryu, Jae-Weon (National Institute of Animal Science, RDA) Choi, Eun-Ji (National Institute of Animal Science, RDA) Son, Jung-Ho (Noah Biotech Inc.)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제32권 제2호
발행연도
2008.1
수록면
123 - 126 (4page)

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The objective of this study was to determine the potential hazardous effects of sorting process by flowcytometry on the quality of boar spermatozoa by flowcytometer. Freshly collected boar semen was diluted and divided into two groups; control none sorted and sorted. Sperms in sorted group were processed with flowcytometer for cell sorting with $100\;{\mu}M$ nozzle under the 20 psi pressure. Measurements on each parameter were made at two time points, 0hr (right after sorting) and 24 hr post sorting. Although there was a tendency of lower viability in sorted group than none sorted control group, the percentage of live cells in control ($75.83{\pm}6.92\;&\;59.53{\pm}10.34$) was not significantly different from sorted ($59.70{\pm}7.37\;&\;43.97{\pm}3.76$) at both 0 and 24 hr post sorting. However, sorted sperm showed significantly lower mitochondrial function compared to the control at both 0 h ($79.37{\pm}3.22\;vs.\;63.50{\pm}10.05$) and 24 hr ($67.27{\pm}3.22$ vs. $46.97{\pm}5.37$) time points (p<0.007). Sperm DNA fragmentation rate was significantly lower in control ($22.0{\pm}7.04$) than that of sorted ($32.27{\pm}7.49$) at 24 hr time point (p<0.0002). Taken together, these data suggested thatsorting process by flowcytometer may have influenced sperm motility rather than viability. Also high speed sperm sorting by flowcytometer has significant effects on DNA fragmentation on elapsed time after sorting.

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