지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 696 - 700 (5page)
이용수
초록· 키워드
오류제보하기
A quick and reliable method for identifying meat origin is developed to ensure species origin of livestock products for consumers. The present study examined the identification of meat sources (duck, chicken, goat, deer, pig, cattle, sheep, and horse) using PCR by exploiting the mitochondrial 12S rRNA and mitochondrial cytochrome b genes. Species-specific primers were designed for some or all mitochondrial 12S rRNA nucleotide sequences to identify meat samples from duck, chicken, goat, and deer. Mitochondrial cytochrome b genes from pig, cattle, sheep, and horse were used to construct species-specific primers, which were used to amplify DNA from different meat samples. Primer sets developed in this study were found to be superior for detecting meat origin when compared to other available methods, for which the discrimination of meat origin was not equally applicable in some cases. Our new development of species-specific primer sets could be multiplexed in a single PCR reaction to significantly reduce the time and labor required for determining meat samples of unknown origin from the 8 species. Therefore, the technique developed in this study can be used efficiently to trace the meat origin in a commercial venture and help consumers to preserve their rights knowing origin of meat products for social, religious or health consciousness.
목차
등록된 정보가 없습니다.
참고문헌 (8)
참고문헌 신청
Chen, S. Y. / 2012 / Species identification of ten common farm animals based on mitochondrial 12S rRNA gene polymorphisms / Anim. Biotechnol 23:213~220
Chikuni, K. / 1994 / Polymerase chain reaction assay for detection of sheep and goat meats / Meat Sci 37:337~345
Chikuni, K. / 1994 / Sequencing of mitochondrial cytochrome b genes for the identification of meat species / J. Anim. Sci. Technol 65:571~579
Fei, S. / 1996 / Species identification of meats and meat products by PCR / J. Anim. Sci. Technol 67:900~905
Girish P. S. / 2004 / Sequence analysis of mitochondrial 12S rRNA gene can identify meat species / Meat Sci 66:551~556
Chikuni, K. / 1994 / Polymerase chain reaction assay for detection of sheep and goat meats / Meat Sci 37:337~345
Chikuni, K. / 1994 / Sequencing of mitochondrial cytochrome b genes for the identification of meat species / J. Anim. Sci. Technol 65:571~579
Fei, S. / 1996 / Species identification of meats and meat products by PCR / J. Anim. Sci. Technol 67:900~905
Girish P. S. / 2004 / Sequence analysis of mitochondrial 12S rRNA gene can identify meat species / Meat Sci 66:551~556
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