Effect of Insulin Supplement on Development of Porcine Parthenogenetic Embryos

Yu, Youngkwang; Roy, Pantu Kumar; Min, Kyuhong; Na, Seungwon; Lee, Euncheol; Kim, Ghangyong; Fang, Xun; Salih, MB; Cho, Jongki

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자료유형: 학술저널

저자정보: Yu, Youngkwang (College of Veterinary Medicine, Chungnam National University) Roy, Pantu Kumar (College of Veterinary Medicine, Chungnam National University) Min, Kyuhong (College of Veterinary Medicine, Chungnam National University) Na, Seungwon (College of Veterinary Medicine, Chungnam National University) Lee, Euncheol (College of Veterinary Medicine, Chungnam National University) Kim, Ghangyong (College of Veterinary Medicine, Chungnam National University) Fang, Xun (College of Veterinary Medicine, Chungnam National University) Salih, MB (College of Veterinary Medicine, Chungnam National University) Cho, Jongki (College of Veterinary Medicine, Chungnam National University)

저널정보: 한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제31권 제2호

발행연도: 2016.1

수록면: 123 - 129 (7page)

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This study is performed to evaluate the effect of insulin in the porcine parthenogenetic embryo development. In porcine embryo culture, insulin is helpful factor in the process of embryo development. To identify this, insulin is used in pig embryos development. Therefore, this study was performed to investigate the effect of insulin on early embryonic development in pigs. For that, insulin positive or negative (0, 10 ug/mL) was supplemented in the porcine IVM media and then compared two groups divided by the cytoplasm of the black groups and white ring groups based on the distribution of lipid material of the cell cytoplasm in microscope. In maturation rates of porcine oocytes, significant higher black group rates were shown in the insulin positive groups compared with other groups ($56.0{\pm}2.1$ vs $46.2{\pm}0.3$). In the embryo culture, black groups were showed the significant higher cleavage rates ($82.1{\pm}0.8$, $78.3{\pm}0.1$ vs $63.2{\pm}0.3$, $63.4{\pm}0.0$), and blastocyst formation rates ($15.5{\pm}3.6$, $16.6{\pm}0.4$ vs $11.7{\pm}1.3$, $7.4{\pm}0.2$) regardless of whether the addition of insulin. Also, black groups were showed higher cell number of blastocyst ($33.2{\pm}2.5$, $35.5{\pm}2.6$ vs $31.2{\pm}2.1$, $31.3{\pm}2.2$). In conclusion, supplement of insulin producing black group in vitro maturation, it was effective in vitro maturation and embryonic development of pig embryos.

#Porcine #Embryo #in vitro production #Insulin

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