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논문 기본 정보

자료유형
학술저널
저자정보
(순천향대학교) (순천향대학교) (순천향대학교)
저널정보
한국생물공학회 KSBB Journal KSBB Journal Vol.35 No.3(Wn.174)
발행연도
수록면
199 - 207 (9page)
DOI
10.7841/ksbbj.2020.35.3.199

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초록· 키워드

Our previous research demonstrated the characteristics and fibrinolytic activities of NaCl- and capsaicin-resistant bacterium Bacillus amyloliquefaciens G-13, isolated from mustard leaf kimchi [1]. In this study, we extended the work to the comparison of various physicochemical factors (e.g., temperature, pH, NaCl, capsaicin, metal ions, and inhibitors) influencing the production of fibrinolytic enzyme by strain G-13. Initially, a time course of the fibrinolytic activity of strain G-13 was measured by the fibrin plate assay, and after 84 hours of incubation, the maximum fibrinolytic activity was about 3.42 times that of plasmin used as the standard. Through a fibrin zymographic assay, four bands (23, 34, 45, and 67 kDa) of fibrinolytic enzyme from strain G-13 were compared in fibrin zymographic gels. The strain could grow and produce the fibrinolytic enzyme in the presence of NaCl (1-10%) or capsaicin (0-300 ㎍/mL), respectively. The optimum pH and temperature for the enzymatic activity were 8 and 35℃. In the study of the effects of metal ions on fibrinolytic activity, K⁺, Ca<SUP>2+</SUP>, and Mg<SUP>2+</SUP> ions increased the activity and maintained it overall, but Fe<SUP>3+</SUP>, Zn<SUP>2+</SUP>, Ba<SUP>2+</SUP>, and Hg<SUP>2+</SUP> ions rapidly decreased the activity of the fibrinolytic enzyme. The enzyme was completely inhibited by phenylmethanesulfonyl fluroide (PMSF), suggesting that it was a serine protease. These results provide important clues for understanding the characteristics of fibrinolytic bacteria by confirming the effects of B. amyloliquefaciens G-13 on various physicochemical factors influencing the production of fibrinolytic enzymes through fibrin zymography.
#Bacillus amyloliqeufaciens G-13 #fibrinolytic enzyme #fibrin zymograpy
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목차

  1. Abstract
  2. 1. INTRODUCTION
  3. 2. MATERIALS AND METHODS
  4. 3. RESULTS AND DISCUSSION
  5. 4. CONCLUSION
  6. REFERENCES

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UCI(KEPA) : I410-ECN-0101-2020-470-001290391