지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.12
- 수록면
- 511 - 516 (6page)
- DOI
- 10.4283/JMAG.2020.25.4.511
이용수
초록· 키워드
오류제보하기
The effects of alternating magnetic field (AMF) stimulation on the proliferation of human liver cancer cells (HepG2) under varying frequencies from 500 ㎐ to 2 ㎑ were investigated. The AMF stimulator generated a magnetic field of 5 mT with a saw tooth pulse waveform, the frequency of alternating pulses was controlled by the duty factor of the digital control circuit. HepG2 cells were cultured in a 6-well plate over a magnetic coil using a cooling system while been kept for 48 hours in an incubator. After magnetic stimulation of 0.5, 1 and 2 ㎑, the proliferation rates of the HepG2 cells saw almost no difference compared to liver cancer cells that were not stimulated. However, the proliferation rates at 1.4 ㎑ were reduced by up to 66 % compared to the non-stimulated cells. In other words, HepG2 cancer cells can be suppressed by magnetic stimulation of a certain frequency. These results are in good agreement with those from analysis of cell viability using MTT assay, a colorimetric assay used for assessing cell metabolic activity. This phenomenon of a suppression frequency may be related with various ionic flows that occur in the ion channels of cell membranes.
목차
1. Introduction
2. Materials and Methods
3. Results and Discussion
4. Conclusion
References
참고문헌 (11)
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