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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.4
- 수록면
- 147 - 147 (1page)
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Fine-tuning gene expression is one of the most important things in both synthetic biology and metabolic engineering. The optimization of the gene expression levels to construct a genetic circuit or microbial cell factories is a major challenge. We created the synthetic toolboxes for fine-tuning gene expression regardless of the coding sequence in Escherichia coli. Firstly, a leader sequence was designed using the computational model(1) to diminish the structural effects of the coding sequence on the secondary structure of the translation initiation region of mRNA. The optimized leader sequence and the 24 expression toolboxes were combined, and their gene expression fine-tuning abilities were evaluated using f ... 전체 초록 보기
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