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Background : The study evaluated the anti-obesity effects of cultivated materials in Jeollabuk-do and Jinan and in order to compare the anti-obesity efficacy of single material and composite material.
Methods and Results : The samples of Morus alba L. (MA) and Aronia melanocarpa (Michx.) Elliott (AR) were extracted 80% ethanol (80% EtOH) twice and freeze-dried powder. The powders were mixed after melt in distilled water. The ability of MA and AR 80% EtOH to inhibit differentiation was measured using a Oil red O staining after treated MDI (IBMX, Dexamethasone, Insulin) in 3T3-L1 cells. MA 80% EtOH was treated concentration of 100, 250, 500 μg/㎖. AR 80% EtOH was treated concentration of 100, 500, 1000 μg/㎖. The mRNA expression of C57BL/6J liver and epididymis fat related to anti-obesity were measured by real-time PCR. protein expression of C57BL/6J liver related to anti-obesity were measured by western blot. The extraction yield of MA 80% EtOH was 19.1% and AR 80% EtOH was 46.2%. In t ... 전체 초록 보기
Methods and Results : The samples of Morus alba L. (MA) and Aronia melanocarpa (Michx.) Elliott (AR) were extracted 80% ethanol (80% EtOH) twice and freeze-dried powder. The powders were mixed after melt in distilled water. The ability of MA and AR 80% EtOH to inhibit differentiation was measured using a Oil red O staining after treated MDI (IBMX, Dexamethasone, Insulin) in 3T3-L1 cells. MA 80% EtOH was treated concentration of 100, 250, 500 μg/㎖. AR 80% EtOH was treated concentration of 100, 500, 1000 μg/㎖. The mRNA expression of C57BL/6J liver and epididymis fat related to anti-obesity were measured by real-time PCR. protein expression of C57BL/6J liver related to anti-obesity were measured by western blot. The extraction yield of MA 80% EtOH was 19.1% and AR 80% EtOH was 46.2%. In t ... 전체 초록 보기
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