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(Daegu Catholic University School of Medicine) (Daegu Catholic University School of Medicine) (Daegu Catholic University School of Medicine) (Chung-Ang University) (Daegu Catholic University School of Medicine)
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한국미생물학회 한국미생물학회 학술대회논문집 2020년도 ANNUAL MEETING OF THE MICROBIOLOGICAL SOCIETY OF KOREA
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    초록·키워드

    Lipid A and core O-antigen polysaccharide (OP) are the main components of lipopolysaccharide (LPS) in Gram-negative bacteria, which may play an important role in host immune responses. However, no suitable method has been developed for the preparation of single lipid A and core OP fractions to evaluate their effects. To solve this problem, we developed an efficient protocol for LPS extraction from an attenuated S. Typhimurium mutant strain in a safe manner, separation of deacylated lipid A and core OP using a Sep-Pak tC18 cartridge after trifluoroacetic acid hydrolysis of LPS at 50°C, and optimization of size-exclusion chromatography for preparative-scale purification of single lipid A and cor ... 전체 초록 보기

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